Protein Info for Pf6N2E2_5102 in Pseudomonas fluorescens FW300-N2E2
Annotation: GTP-binding protein Obg
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to OBG_PSEPF: GTPase Obg (obg) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K03979, GTP-binding protein (inferred from 100% identity to pba:PSEBR_a4863)MetaCyc: 66% identical to GTPase ObgE (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]
Predicted SEED Role
"GTP-binding protein Obg"
MetaCyc Pathways
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
- pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
- UTP and CTP dephosphorylation I (5/7 steps found)
- UTP and CTP dephosphorylation II (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.6.1.15
Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165ZN90 at UniProt or InterPro
Protein Sequence (407 amino acids)
>Pf6N2E2_5102 GTP-binding protein Obg (Pseudomonas fluorescens FW300-N2E2) MKFVDEVSIRVKAGDGGNGCMSFRREKFIENGGPNGGDGGDGGSIYMLADENLNTLVDYR YTRHFDAERGSNGGSTDCTGKKGEDLILRVPVGTTVIDSATQEVIGDLTKAGQRLLVAHG GWHGLGNTRFKSSTNRAPRQTTPGKPGEQRDLKLEMKVLADVGLLGLPNAGKSTFIRSVS AAKPKVADYPFTTLVPNLGVVSVDRWKSFVIADIPGLIEGASEGAGLGIRFLKHLSRTRL LLHLVDMAPLDETSAADAAEVIVNELTKFSPALAERDRWLVLNKCDQILEEEHEERVKEI VDRLEWEGPVYVISAIAKEGTERLCHDIMRYLEDRADRLANDPAYKEELAELDQRIEDEA RAQLQALDDQRALRRSGVKSVHDIGDDDWDEEDVDDEDGPEIIYVRD