Protein Info for Pf6N2E2_5076 in Pseudomonas fluorescens FW300-N2E2

Annotation: DNA repair protein RadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 TIGR00416: DNA repair protein RadA" amino acids 1 to 450 (450 residues), 671.2 bits, see alignment E=3.5e-206 PF18073: Rubredoxin_2" amino acids 8 to 35 (28 residues), 38.2 bits, see alignment (E = 2.3e-13) PF06745: ATPase" amino acids 79 to 146 (68 residues), 40.3 bits, see alignment E=6.1e-14 PF13481: AAA_25" amino acids 80 to 221 (142 residues), 45.7 bits, see alignment E=1.5e-15 PF13541: ChlI" amino acids 351 to 429 (79 residues), 32.3 bits, see alignment E=2e-11 PF05362: Lon_C" amino acids 370 to 452 (83 residues), 27.8 bits, see alignment E=4.5e-10

Best Hits

Swiss-Prot: 91% identical to RADA_PSEAE: DNA repair protein RadA (radA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K04485, DNA repair protein RadA/Sms (inferred from 100% identity to pba:PSEBR_a4888)

Predicted SEED Role

"DNA repair protein RadA" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GTU8 at UniProt or InterPro

Protein Sequence (455 amino acids)

>Pf6N2E2_5076 DNA repair protein RadA (Pseudomonas fluorescens FW300-N2E2)
MAKAKRMYGCTECGSTFPKWAGQCSECGAWNTLTETMVESGGAAAPTGRTGWAGQQAQIK
TLAEVSVEEIPRFSTASGELDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLATRMP
ALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIATARLEKPKVMVIDSIQTIFT
EQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATW
EGTRPMLVEVQALVDDSHLANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVG
GVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSGEVRPVPSGQERLKEAAKHGF
KRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE