Protein Info for Pf6N2E2_5036 in Pseudomonas fluorescens FW300-N2E2

Annotation: Cobalt-zinc-cadmium resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 transmembrane" amino acids 16 to 35 (20 residues), see Phobius details amino acids 42 to 65 (24 residues), see Phobius details amino acids 84 to 104 (21 residues), see Phobius details amino acids 116 to 136 (21 residues), see Phobius details amino acids 160 to 177 (18 residues), see Phobius details amino acids 183 to 200 (18 residues), see Phobius details TIGR01297: cation diffusion facilitator family transporter" amino acids 15 to 289 (275 residues), 207.3 bits, see alignment E=1.5e-65 PF01545: Cation_efflux" amino acids 17 to 208 (192 residues), 139 bits, see alignment E=1.7e-44 PF16916: ZT_dimer" amino acids 212 to 288 (77 residues), 79.9 bits, see alignment E=1.2e-26

Best Hits

Swiss-Prot: 49% identical to FIEF_SALA4: Cation-efflux pump FieF (fieF) from Salmonella agona (strain SL483)

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a4931)

MetaCyc: 48% identical to Zn2+/Fe2+/Cd2+ exporter (Escherichia coli K-12 substr. MG1655)
RXN0-6; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A2N2 at UniProt or InterPro

Protein Sequence (297 amino acids)

>Pf6N2E2_5036 Cobalt-zinc-cadmium resistance protein (Pseudomonas fluorescens FW300-N2E2)
MTTSTEHARLLRLATRASVAVALILVVAKALAWWLSGSVSMLAGLTDSALDGVTSLLNLL
AVHYALRPADDDHRYGHGKAESLAGMAQALFIGGSALLIALQAFERLKNPVPVDAPWLSV
GVIVFSLGLTLALLALQHRVIRATGSNAVRADSLHYRSDLLLNGSILVALVLAGFGWYQL
DAWFGLGIAIYILWSAVQIARESFAVLMDQELPPDVSQHMLELACAVPGVVGAHDLRTRI
SGNHWFVQLHLELPGELTLSVAHGISDQAADAIHRAYPKAEVLVHADPSEVVKRANA