Protein Info for Pf6N2E2_4993 in Pseudomonas fluorescens FW300-N2E2

Annotation: Gamma-glutamyl phosphate reductase (EC 1.2.1.41)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 6 to 278 (273 residues), 46.8 bits, E=8.2e-17
  • amino acids 291 to 367 (77 residues), 27.9 bits, E=4.4e-11
• TIGR00407: glutamate-5-semialdehyde dehydrogenase
  • amino acids 8 to 399 (392 residues), 479.7 bits, E=3.3e-148

Best Hits

Swiss-Prot: 93% identical to PROA_PSEPF: Gamma-glutamyl phosphate reductase (proA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00147, glutamate-5-semialdehyde dehydrogenase [EC: 1.2.1.41] (inferred from 99% identity to pba:PSEBR_a4968)

MetaCyc: 45% identical to glutamate-5-semialdehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate-5-semialdehyde dehydrogenase. [EC: 1.2.1.41]

Predicted SEED Role

"Gamma-glutamyl phosphate reductase (EC 1.2.1.41)" in subsystem Proline Synthesis (EC 1.2.1.41)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.41

Sequence Analysis Tools

See A0A160A4N7 at UniProt or InterPro

Protein Sequence (415 amino acids)

>Pf6N2E2_4993 Gamma-glutamyl phosphate reductase (EC 1.2.1.41) (Pseudomonas fluorescens FW300-N2E2)
MTRLGRAARQASRIIGRASTAQKNRALQAAANALDAARAELSAANELDLAAGRANGLEPA
MLERLALTPARIDGMIVGLRQVAALPDPVGAIRDMSYRPSGIQVGKMRVPLGVVGIIYES
RPNVTIDAASLCLKSGNATILRGGSEAIHSNRAIAACIQRGLAEADLPAAVVQVVETTDR
AAVGALITMPEFVDVIVPRGGKGLIERVSRDARVPVIKHLDGICHVYVSAHAELPKAQRI
AFNAKTYRYGICGAMETLLVDQAVAKDFLPSMAAQFREKGVELRGCERTRAIIEAVAATE
EDWDTEYLAPILSIRVVDGLDEAIEHINRHGSHHTDSIVSEHLGETRRFVAEVDSASVMI
NTPTCFADGFEYGLGAEIGISTDKLHARGPVGLEGLTCEKYIVVGDGQLRGQEPV