Protein Info for Pf6N2E2_4992 in Pseudomonas fluorescens FW300-N2E2
Annotation: DNA-3-methyladenine glycosylase II (EC 3.2.2.21)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 87% identical to 3MGH_PSEPF: Putative 3-methyladenine DNA glycosylase (Pfl01_4975) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K03652, DNA-3-methyladenine glycosylase [EC: 3.2.2.21] (inferred from 99% identity to pba:PSEBR_a4969)Predicted SEED Role
"DNA-3-methyladenine glycosylase II (EC 3.2.2.21)" in subsystem DNA Repair Base Excision (EC 3.2.2.21)
Isozymes
Compare fitness of predicted isozymes for: 3.2.2.21
Use Curated BLAST to search for 3.2.2.21
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A3Z1 at UniProt or InterPro
Protein Sequence (225 amino acids)
>Pf6N2E2_4992 DNA-3-methyladenine glycosylase II (EC 3.2.2.21) (Pseudomonas fluorescens FW300-N2E2) MTDLPSPNALPHAFFDRDAQVLAKDLLGKVIRHKVGDLWLSARIIETEAYYFAEKGSHAS LGYTEKRKALFLDGGHIYMYYARGGDSLNFSAQGPGNAVLIKSAYPWVDALSGPASLAQM LLNNPDAQGRPRTPQKLCAGQTLLCKALGLKVPDWDAKRFDPERLLVEDVGVPTVNVIQT TRLGIPLGRDEHLPYRFVDAAYAPWCTRNPLRRGQVEGRDYFLLG