Protein Info for Pf6N2E2_498 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): Dihydropyrimidinase (EC 3.5.2.2)
Rationale: Specifically important for utilizing Cytosine. Automated validation from mutant phenotype: the predicted function (3.5.2.2) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Dihydropyrimidinase (EC 3.5.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02033: dihydropyrimidinase
  • amino acids 3 to 455 (453 residues), 589.8 bits, E=2e-181
• PF01979: Amidohydro_1
  • amino acids 50 to 437 (388 residues), 91 bits, E=9.6e-30
• PF07969: Amidohydro_3
  • amino acids 356 to 435 (80 residues), 35.5 bits, E=8.9e-13

Best Hits

Swiss-Prot: 86% identical to HYDA_PSEAE: D-hydantoinase/dihydropyrimidinase (dht) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 99% identity to pba:PSEBR_a3367)

MetaCyc: 49% identical to dihydropyrimidinase (Arabidopsis thaliana col)
Dihydropyrimidinase. [EC: 3.5.2.2]

Predicted SEED Role

"Dihydropyrimidinase (EC 3.5.2.2)" in subsystem Hydantoin metabolism or Pyrimidine utilization (EC 3.5.2.2)

MetaCyc Pathways

• thymine degradation (3/3 steps found)
• uracil degradation I (reductive) (3/3 steps found)
• superpathway of pyrimidine ribonucleosides degradation (3/5 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.2

Sequence Analysis Tools

See A0A159ZRC5 at UniProt or InterPro

Protein Sequence (479 amino acids)

>Pf6N2E2_498 Dihydropyrimidinase (EC 3.5.2.2) (Pseudomonas fluorescens FW300-N2E2)
MSLLIRGATVITHDESYRADVFCADGVIKAIGDNLDVPAAVEVLDGGGQYLMPGGIDPHT
HMQLPFMGTVASEDFFSGTAAGLAGGTTSIIDFVIPNPQQSLMEAFHQWRGWAEKSASDY
GFHVAITWWSEQVREEMAELVNHHGVNSFKHFMAYKNAIMAADDTLVASFERCLELGAVP
TVHAENGELVYHLQRKLLAQGITGPEAHPLSRPSQVEGEAASRAIRIAETLGTPLYLVHV
STKEALDEITYARSKGQPVYGEVLAGHLLLDDSVYRDPDWQTAAGYVMSPPFRPRGHQEA
LWHGLQSGNLHTTATDHCCFCAEQKAAGRDDFSKIPNGTAGIEDRMAVLWDEGVNSGKLS
MHDFVALTSTNTAKIFNLYPRKGAIRVGADADLVLWDPQGSRTISAKTHHQQVDFNIFEG
KTVRGVPSHTISQGRLVWADGDLRAERSAGRYIERPAYPAVFDLLSKRAELNKPTAVKR