Protein Info for Pf6N2E2_4977 in Pseudomonas fluorescens FW300-N2E2

Annotation: Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 PF06751: EutB" amino acids 13 to 441 (429 residues), 728.5 bits, see alignment E=1.4e-223

Best Hits

Swiss-Prot: 51% identical to EUTB_ECO57: Ethanolamine ammonia-lyase heavy chain (eutB) from Escherichia coli O157:H7

KEGG orthology group: K03735, ethanolamine ammonia-lyase large subunit [EC: 4.3.1.7] (inferred from 99% identity to pba:PSEBR_a4984)

MetaCyc: 51% identical to ethanolamine ammonia-lyase subunit alpha (Escherichia coli K-12 substr. MG1655)
Ethanolamine ammonia-lyase. [EC: 4.3.1.7]

Predicted SEED Role

"Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7)" in subsystem Ethanolamine utilization (EC 4.3.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.7

Use Curated BLAST to search for 4.3.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GT46 at UniProt or InterPro

Protein Sequence (464 amino acids)

>Pf6N2E2_4977 Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7) (Pseudomonas fluorescens FW300-N2E2)
MAAFAHSVGAQTYRFDSLKDLMAKASPARSGDFLAEVAALNDGERVAAQMALADLPLSHF
LQEMLIPYEVDEVTRLIVDTHDKHAFATVSHLTVGGFRDWLLSDAADEQSLRALAPGLTP
EMVAAVSKIMRVQDLVLVAQKIRVVTKFRGTLGLRGRLSTRLQPNHPTDEPAGIAASILD
GLLFGNGDAMIGINPATDSTASICAMLEMLDAIIQRYDIPTQGCVLTHVTTSIEAANRGV
PLDLVFQSIAGTEAANASFGINLNVLKEGYDAGLSLNRGTLGNNLMYFETGQGSALSANA
HHGVDQQTCETRAYAVARHFKPFLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGVP
MGCDICYTNHAEADQDDMDTLLTLLGVAGINFIMGIPGSDDIMLNYQTTSFHDALYARQT
LGLKPAPEFETWLANMGIFTQADGRVRFGDNLPPAFRHALAHLG