Protein Info for Pf6N2E2_4976 in Pseudomonas fluorescens FW300-N2E2
Annotation: Ethanolamine ammonia-lyase light chain (EC 4.3.1.7)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to EUTC_PSEF5: Ethanolamine ammonia-lyase light chain (eutC) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
KEGG orthology group: K03736, ethanolamine ammonia-lyase small subunit [EC: 4.3.1.7] (inferred from 96% identity to pba:PSEBR_a4985)Predicted SEED Role
"Ethanolamine ammonia-lyase light chain (EC 4.3.1.7)" in subsystem Ethanolamine utilization (EC 4.3.1.7)
MetaCyc Pathways
- ethanolamine utilization (5/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.7
Use Curated BLAST to search for 4.3.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A0Y9 at UniProt or InterPro
Protein Sequence (274 amino acids)
>Pf6N2E2_4976 Ethanolamine ammonia-lyase light chain (EC 4.3.1.7) (Pseudomonas fluorescens FW300-N2E2) MEKKPVDPQNPWLNLRNLTPARIALGRTGTSLPTQAQLDFQYAHAQARDAVHLAFDHQGI RAQLSERGRESLLLHSAAADRHSYLQRPDLGRRLDDVSAQVLDDYAKAHPGGVDLAIVVA DGLSALAVHRHTLPFLARLEEQIVADGWSVSPVILVEQGRVAVADEVAERLGAKMSVILI GERPGLSSPDSLGLYFTYAPKVGLTDAYRNCISNVRLEGLSYGMAAHRLIYLMREACRRQ LSGVNLKDEAQVHTLESENTADMKGNFLLMPPRN