Protein Info for Pf6N2E2_497 in Pseudomonas fluorescens FW300-N2E2

Annotation: Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14691: Fer4_20
  • amino acids 21 to 127 (107 residues), 116.5 bits, E=2.2e-37
• PF03486: HI0933_like
  • amino acids 142 to 176 (35 residues), 24 bits, 6.2e-09
• PF07992: Pyr_redox_2
  • amino acids 142 to 437 (296 residues), 122.2 bits, E=1.2e-38
• PF00890: FAD_binding_2
  • amino acids 143 to 177 (35 residues), 24.5 bits, 6e-09
• PF13450: NAD_binding_8
  • amino acids 145 to 177 (33 residues), 36.1 bits, see alignment (E = 2.7e-12)

Best Hits

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 97% identity to pba:PSEBR_a3368)

Predicted SEED Role

"Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism" in subsystem Pyrimidine utilization

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14

Sequence Analysis Tools

See A0A159ZRR2 at UniProt or InterPro

Protein Sequence (455 amino acids)

>Pf6N2E2_497 Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism (Pseudomonas fluorescens FW300-N2E2)
MIQPLSHLPHSQEDPATLAARFSDLAPPLNARQAHLEASRCLYCYDAPCVNACPSEIDIP
SFIRNIHQDNVQGAAQKILSANILGGSCARVCPTEILCQQACVRNNVHECAPVLIGQLQR
YAVDNAHFSEHPFKRAATTGKRIAVVGAGPAGLSCAHRSAMHGHDVVVFEAREKAGGLNE
YGIAKYKLVDDYAQRELDFLLEIGGIEIRHGQKLGENLSLSDLHQQFDAVFLGLGLAASK
QLGLSDEQAPGLLAATDYIRELRQADDLSQLPLADRCIVLGAGNTAIDMAVQMARLGARD
VNLVYRRGLEDMGATVHEQDIAKANQVRLLTWAQPQQVLLDDTGNVRGMRFSRTHLENGR
LVIGTDTFDLPADAIFKAIGQAFDDNALVDPLARELKRQDGRILVDEHLRTSIPGVYAGG
DCTSLDQDLTVQAVQHGKLAAEAINAQLMLNVEAA