Protein Info for Pf6N2E2_4939 in Pseudomonas fluorescens FW300-N2E2

Annotation: alpha-2-macroglobulin domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1517 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF01835: MG2" amino acids 328 to 424 (97 residues), 67.4 bits, see alignment 2.4e-22 PF07703: A2M_BRD" amino acids 648 to 794 (147 residues), 54.8 bits, see alignment E=2.6e-18 PF00207: A2M" amino acids 859 to 947 (89 residues), 62.7 bits, see alignment 4.6e-21

Best Hits

Swiss-Prot: 70% identical to A2MGH_PSEAE: Alpha-2-macroglobulin homolog (PA4489) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A2G5 at UniProt or InterPro

Protein Sequence (1517 amino acids)

>Pf6N2E2_4939 alpha-2-macroglobulin domain protein (Pseudomonas fluorescens FW300-N2E2)
MLRLCSKLPLLFALLLAPLVNAEDTVAPSGYTPVAGESFFLLADSSFASDEQAMVRLEAP
GRDYRRFRMEPYGGADIRVYRIDKPLDFLKRQKNLHRVVSDGQFKGEGLSNTLAYLWDNW
YRKSRRVMQRAFSYESRQQVTEEVPELKIGDALVAPTPYEAPPQFALIPGLPVVSQFRYP
LWEAKPIQPPAGVNLAGSSSEFVSVAPGNVYVPLGQLKPGLYLVEALIGKYRATTMVFVS
NTVAVSKIAGDELLVWAARKHEGRSVPKVNVLWTDGLGVMSSGATDENGLLRLKHVSPER
SFVIGEDEEGGVFVSENFYYDSEIYDTKLYAFTDRPLYRPGDWVSLKIVGREFKNARDSV
QPSAADVNVSVLDATGTELQTLALKLDSKAGTQGRFQLPENAVAGGYELRLRYKDQLYSS
AFRVAEYIKPHFEIALNLAKQDYRTGEPVKGNLVLLYPDGKPVVNATVSLSLRAQQLSMV
DNELQYLGQFPVELTSSEVTTDAKGNATLDLPAADKPSRYTLTVFASDGAAYRVKTTKEI
LIDRGAANFRLSAPQRFSAAGQKVSFSYANEGGNEQAKAVTPGSYAWVRLEDQSTGEGKL
AAKDKGFSLTFDRPGTYNLTLKDDHGRVIGAAAHAVTGEGIKAVPGTVEIVFDKPEYKTG
DEALALITFPEPVSDALLSLERDKVEATALLSKGGDWLKMEKLSDTQYRARIAVRDNFAP
NLTFSVLYTKGGQYSFQNAGIKVITPQIDVAVVTDKATYRPGDTVTVDLSTQFSGKAIPA
HLTVSVVDEMVYALQPEVAPTIDQFFYHPRRNNVRTSASLSFISYDVALPGSPGAPGKAN
RSERGVKVLERPRREDVDTAAWQPELVTDANGKARFTFKMPDSLTRWRITAKAIADDGQV
GQKKQFIASEKPLYLKWSGPTRFRTGDKPQLGLFAFSQSEKPLKAELLIRYAGAEQRMVV
DLKNGINYVALPALELSSGDLNVQLQLNGETQDALAMRLNASGNGWQVTQSQRLDVASGD
TPLTLPADASDIRLRLDDSPQALFRSALDDLLSYPYGGVEQTASRLLPLSIAYPTLASNP
QIRDRLRLIMQNSRLRLVQMAGPSASFTWWGQDGEPDAFLTAYAYYADWHASKALDLSLP
PEHWQRVLEVYAKQAGDTPLLQRALILSFAKQMQLPVNTLLSGLMDDLAKASEDSSESVL
DSGEDSLVMYAPDSALGLASARVLTASLAKQAKVPLPAAFSRQADAAQQQLELSSQPFVE
ALVLSLQGFDQARATALLERVLPQQSTLERALALTWLQRSIEQAAPAVALAPGEGWAAKQ
GATGETYWQWQGAQLPTMLTLTGAQERPLQAALSFQSQQPPAAPMAVSITRRLSRLVPGD
EAFTFKLEAVGNKPLSSDSLYLDEVIINSKAPTPLRYGMLEVPLPPGADVERTTWGIQLM
GKADSEPMSLEKARFEPGQMAYAVPVDALSGELRLRHLVRFSQKGQFTLPPVRFTQVYAP
QHQAREQKPALGQVTVN