Protein Info for Pf6N2E2_4919 in Pseudomonas fluorescens FW300-N2E2

Annotation: Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 PF00383: dCMP_cyt_deam_1" amino acids 11 to 108 (98 residues), 76.3 bits, see alignment E=2.3e-25 PF14437: MafB19-deam" amino acids 13 to 150 (138 residues), 51.2 bits, see alignment E=1.8e-17 TIGR00326: riboflavin biosynthesis protein RibD" amino acids 15 to 367 (353 residues), 402.6 bits, see alignment E=1.5e-124 TIGR00227: riboflavin-specific deaminase C-terminal domain" amino acids 154 to 369 (216 residues), 180.1 bits, see alignment E=4.5e-57 PF01872: RibD_C" amino acids 155 to 365 (211 residues), 167.8 bits, see alignment E=4.1e-53

Best Hits

KEGG orthology group: K11752, diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase [EC: 1.1.1.193 3.5.4.26] (inferred from 98% identity to pba:PSEBR_a5040)

Predicted SEED Role

"Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)" in subsystem Riboflavin, FMN and FAD metabolism (EC 1.1.1.193, EC 3.5.4.26)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.193

Use Curated BLAST to search for 1.1.1.193 or 3.5.4.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3U8 at UniProt or InterPro

Protein Sequence (377 amino acids)

>Pf6N2E2_4919 Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193) (Pseudomonas fluorescens FW300-N2E2)
MTGSAQQAVLDAHYMARALELARRGHYTTHPNPRVGCVIVRDGQIVGEGWHIRAGEPHAE
VHALRAAGEQARGATAYVTLEPCSHHGRTPPCADALVNAGVARVVAAMQDPNPEVAGRGL
QRLAQAGIATESGVLEGEARKLNEGFLKRMEQGLPFVRVKLAMSLDGRTAMESGESQWIT
GPAARSAVQRLRAQASVVLTGADTVLADNARLTVRADELGLDAEQTALVMSRPPLRVLVD
GRLRVPLDAAFFKAGPALVATCMAVEEQYANGPECMIVAGDDGQVDLRRLLVALAARDVN
EVLVEAGPRLAGAFARQGLVDEFQIFIAGKFLGSSARPLLDWPLAQMKDAPQLKITEIRA
VGDDWRVIAVPVPPASV