Protein Info for Pf6N2E2_4904 in Pseudomonas fluorescens FW300-N2E2

Annotation: Potassium efflux system KefA protein / Small-conductance mechanosensitive channel

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 683 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details transmembrane" amino acids 94 to 116 (23 residues), see Phobius details amino acids 136 to 158 (23 residues), see Phobius details amino acids 166 to 192 (27 residues), see Phobius details amino acids 212 to 231 (20 residues), see Phobius details amino acids 238 to 259 (22 residues), see Phobius details amino acids 293 to 312 (20 residues), see Phobius details amino acids 323 to 344 (22 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details amino acids 407 to 428 (22 residues), see Phobius details amino acids 454 to 473 (20 residues), see Phobius details amino acids 479 to 497 (19 residues), see Phobius details PF21088: MS_channel_1st" amino acids 459 to 498 (40 residues), 33 bits, see alignment 6.9e-12 PF00924: MS_channel_2nd" amino acids 500 to 565 (66 residues), 47.8 bits, see alignment E=1.9e-16 PF21082: MS_channel_3rd" amino acids 572 to 654 (83 residues), 21 bits, see alignment E=5.5e-08

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a5054)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZMH8 at UniProt or InterPro

Protein Sequence (683 amino acids)

>Pf6N2E2_4904 Potassium efflux system KefA protein / Small-conductance mechanosensitive channel (Pseudomonas fluorescens FW300-N2E2)
LLLFLLALTGTSAYGTAAVPGTSKPDEPPAEPAPLVQGGLLGAISSSIDDVQDKLDLNQS
LVDAWRLRADRAADEVDRLVDQTSRSSWRVAGDFLLLSSVWIGAFALIWSGARLLLRHLG
RRRWLRTRQRLRDLLGYLLPYTAAALICLPLTLYVSHFLQVSVGRALALCFAYATSSGIF
STSVLLCVIVMFNAGHKRRAVAMIRRFSPRPLFMIGFLAALSDALTSPQIARQLGGNITS
SIAVFTGLFASVIFGWLVIRMRRPVAHLIRNRPLAQRLKQPALQESLRIFSGLWYWPILL
MVLVSAVSLIGVGEDNQKALRCALFTTILLIATVFLSTVFQHVFKSPKAETIQRNSAYKG
RLLSLLHALLRIVMAVVFIEVLGRIWGISLFEFASRNAIGRAISDSLSRIGLIFLMTWLT
WVVLDTAIQEALKPPLNKRGARQPSTRVKTILPLLRNAAKIILVVICAITTMANLGINVA
PLLAGAGVVGLAIGFGSQQLVQDVITGLFIIIEDTLSIGDWVVLSSGHAGTVEGLTIRTL
RLRDGKGFVHSVPFGQIKAVTNQSRQFAFAFFSVQFTYDTDVDQAIELIREAGRSIAEDP
FLKFNLQGPLDVFGVDKMDLNGVVLTAQFRTVSGGQYAVSRAFNQQLKKLVDNHPAVHFA
QTYPQQVMLPKRFVEEPKESGES