Protein Info for Pf6N2E2_4808 in Pseudomonas fluorescens FW300-N2E2

Annotation: Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 922 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF03968: LptD_N" amino acids 157 to 287 (131 residues), 43.1 bits, see alignment E=4.6e-15 PF04453: LptD" amino acids 399 to 828 (430 residues), 387.8 bits, see alignment E=7.3e-120

Best Hits

Swiss-Prot: 81% identical to LPTD_PSEF5: LPS-assembly protein LptD (lptD) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K04744, LPS-assembly protein (inferred from 92% identity to pba:PSEBR_a5135)

Predicted SEED Role

"Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A0N2 at UniProt or InterPro

Protein Sequence (922 amino acids)

>Pf6N2E2_4808 Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor (Pseudomonas fluorescens FW300-N2E2)
MALKSPAFRKKFPLLVTGSLLALQPLASSFVVAAQQYDCSVSASGAWDCAPKTPAAALPP
RPVHDGSAVSDSGAAPADSSSGEEAGEKPMLVTEAKGRGLKSRSADYSHLDWVPRENLTP
AQLAETGPYCSGAYIEPTRPGMNDKTAKSDAPTFLGAKASRYQQEEQVATLAGDVVMRQG
SMQVEADEASLYQAENRGELSGNVRVRDNGALIVGDHADVQLDTGEAKVDNAEYVMHKSR
IRGNALYAKRAENAIIRLKDGTYTTCEPNSNAWTLKGNNITLNPATGFGTATNVTLRVKD
FPVLYTPYIYFPIDDRRQSGFLPPTIGSGSDTGFLLVTPYYFNLAPNYDATLYPRYMAKR
GLLMEGEFRYLTKSSEGQFGAAYLNDEDDERSGQSDYDKTRYMYNWQHKGGLDSRVLTEV
DYTKISDPYYFQDLKSDQIGIETNDRVNQQGIVSYRGDNYVARLNAQAYQLATISKITPY
NRLPQITFSGALPEHPYGLDFAYKTELVRFDRDLRTGNYTDEDGNVEPWLDTNVRGLARA
NGNRMNLAPVMSLPMTASYGYLKPSLKYQYTQYDLDLDGTGKSVVGNGYDSTPNRSVPIA
SIDSGLYFDRNTQWFGTNYRQTLEPRLFYLYVPEKDQSDIPIFDTSESTFSYSSLFRDNR
FTGSDRVGDENKLSLGVTSRWIEENGFERQRLAVGQAFYFKDRKVQLPGIDFDTREDAKS
DVSPYALEYEYHWNRDWRTTATYNWDPDTRSPRSGSAMFHYQPEDNPNKVINAGYRYRND
QVRYDEATGQWQVGGGDYGRPGDADYVKDYYKIKQHDFSVIWPIVPQWNLISRWQYDYNR
NRTLEAFGGFEYDNCCWKLRLINRYWVDYEEFSQAAPENEKGDHGIFLQIVLKGLGGLTG
AKVESFLDKGIQGYREREDQAF