Protein Info for Pf6N2E2_4803 in Pseudomonas fluorescens FW300-N2E2

Annotation: Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 79% identical to APAH_PSEF5: Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01525, bis(5'-nucleosyl)-tetraphosphatase (symmetrical) [EC: 3.6.1.41] (inferred from 99% identity to pba:PSEBR_a5140)

MetaCyc: 51% identical to diadenosine tetraphosphatase (Escherichia coli K-12 substr. MG1655)
Bis(5'-nucleosyl)-tetraphosphatase (symmetrical). [EC: 3.6.1.41]; Guanosine-5'-triphosphate,3'-diphosphate diphosphatase. [EC: 3.6.1.41, 3.6.1.40]

Predicted SEED Role

"Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)" (EC 3.6.1.41)

MetaCyc Pathways

ppGpp metabolism (6/6 steps found)

KEGG Metabolic Maps

Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.40

Use Curated BLAST to search for 3.6.1.40 or 3.6.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3L3 at UniProt or InterPro

Protein Sequence (291 amino acids)

>Pf6N2E2_4803 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) (Pseudomonas fluorescens FW300-N2E2)
MATYAVGDLQGCLDPLKCLLERVAFDPQKDTLWLVGDLVNRGPQSLETLRFLYGIRQSLV
CVLGNHDLHLLAAWQNIERLKKSDTLSEILDAPDCVELLEWLRQQKLMHYDEARNLALVH
AGIPPQWSLRKALKCAGEVEQALRDDNLFAPYLDGMYGNEPVKWDNDLTGSARLRVITNY
FTRMRFCTRDGKLDLKGKEGIETAPPGYAPWFKHSERKTRNLKIIFGHWAALGGQSDEPG
VFALDTGCVWGSAMTLMNVDTGEQLHCDCDEQGHAKIHHPTTAPLPAGTAV