Protein Info for Pf6N2E2_4801 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): yeaG component of nitrogen-related signalling system (of yeaGH-ycgB)
Rationale: conserved cofitness; yeaG is a protein kinase
Original annotation: Serine protein kinase (prkA protein), P-loop containing

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 PF08298: AAA_PrkA" amino acids 20 to 376 (357 residues), 621.7 bits, see alignment E=3.9e-191 PF06798: PrkA" amino acids 378 to 632 (255 residues), 247.2 bits, see alignment E=2.3e-77

Best Hits

Swiss-Prot: 77% identical to YEAG_ECOL6: Uncharacterized protein YeaG (yeaG) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K07180, serine protein kinase (inferred from 100% identity to pba:PSEBR_a5142)

MetaCyc: 77% identical to protein kinase YeaG (Escherichia coli K-12 substr. MG1655)
2.7.11.d,2.7.11.11,2.7.12.1,2.7.11.12,2.7.11.17,2.7.11.1,2.7.11.13,2.7.11.22,2.7.11.21,2.7.11.34,2.7.11.31 [EC: 2.7.11.1, 2.7.11.11, 2.7.11.12, 2.7.11.13, 2.7.11.17, 2.7.11.21, 2.7.11.22, 2.7.11.31, 2.7.11.34, 2.7.11.d, 2.7.12.1]

Predicted SEED Role

"Serine protein kinase (prkA protein), P-loop containing"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.11.1

Use Curated BLAST to search for 2.7.11.1 or 2.7.11.11 or 2.7.11.12 or 2.7.11.13 or 2.7.11.17 or 2.7.11.21 or 2.7.11.22 or 2.7.11.31 or 2.7.11.34 or 2.7.11.d or 2.7.12.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A2I4 at UniProt or InterPro

Protein Sequence (640 amino acids)

>Pf6N2E2_4801 yeaG component of nitrogen-related signalling system (of yeaGH-ycgB) (Pseudomonas fluorescens FW300-N2E2)
MSIFSHFQQRFESTRQEEYSLQEYLELCKQDRSAYASAAERLLLAIGEPELLDTSTNSRL
SRIFSNKVIRRYPAFADFHGMEECIEQIVSYFRHAAQGLEEKKQILYLLGPVGGGKSSLA
EKLKQLIEKVPFYAIKGSPVFESPLGLFNATEDGAILEEDFGIPRRYLNTIMSPWATKRL
AEFGGDISQFKVVKLYPSILNQIAVAKTEPGDENNQDISALVGKVDIRKLEEFPQNDADA
YSYSGALCRANQGLMEFVEMFKAPIKVLHPLLTATQEGNYNSTEGLGAIPFTGILLAHSN
ESEWHTFRNNKNNEAFIDRIYIVKVPYCLRVSDEVKIYDKLLFNSSLSKAHCAPDTLKML
AQFTVLSRLKEPENSNIYSKMRVYDGENLKDTDPKAKSIQEYRDNAGVDEGMNGLSTRFA
FKILSKVFNFDPHEIAANPVHLLYVLEQQIEQEQFQAETRERYLRFLKEYLAPRYIEFIG
KEIQTAYLESYSEYGQNIFDRYVLYADFWIQDQEYRDPETGEILNRVALNEELEKIEKPA
GISNPKDFRNEIVNFVLRARANNNGKNPTWLSYEKLRVVIEKKMFSNTEDLLPVISFNAK
ASKEDQQKHNDFVTRMVERGYTDKQVRLLSEWYLRVRKSQ