Protein Info for Pf6N2E2_4752 in Pseudomonas fluorescens FW300-N2E2

Annotation: Malate synthase G (EC 2.3.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 4 to 722 (719 residues), 1209.4 bits, E=0
• PF20656: MS_N
  • amino acids 17 to 72 (56 residues), 49.6 bits, 4.5e-17
• PF20658: MSG_insertion
  • amino acids 160 to 235 (76 residues), 110.2 bits, E=9.1e-36
• PF01274: MS_TIM-barrel
  • amino acids 337 to 578 (242 residues), 229.6 bits, E=6.2e-72
• PF20659: MS_C
  • amino acids 591 to 673 (83 residues), 85.4 bits, E=8.5e-28

Best Hits

Swiss-Prot: 93% identical to MASZ_PSEF5: Malate synthase G (glcB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 99% identity to pba:PSEBR_a5188)

MetaCyc: 67% identical to malate synthase G (Mycobacterium tuberculosis H37Rv)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• superpathway of glycol metabolism and degradation (7/7 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glyoxylate cycle (5/6 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• L-arabinose degradation IV (5/8 steps found)
• chitin deacetylation (2/4 steps found)
• D-xylose degradation IV (3/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (1/14 steps found)
• superpathway of pentose and pentitol degradation (15/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See A0A160A0H6 at UniProt or InterPro

Protein Sequence (725 amino acids)

>Pf6N2E2_4752 Malate synthase G (EC 2.3.3.9) (Pseudomonas fluorescens FW300-N2E2)
MTEHVQVGGLQVAKVLFDFVNNEAIPGTGLTADAFWAGADKVIHDLAPKNKALLAKRDDF
QARIDAWHQARTGQAHDAVAYKAFLQDIGYLLPEAADFQATTQNVDDEIARMAGPQLVVP
VMNARFALNASNARWGSLYDALYGTDAISEADGAEKGKGYNKVRGDKVIAFARAFLDEAA
PLAAGSHVDSIAYKIVDGKLVVTLKGGSNSGLRDDAQLIGFQGDASAPTTVLLKHNGLHF
EIQIDASTPVGQTDAAGVKDILMEAALTTIMDCEDSVAAVDADDKVVIYRNWLGLMKGDL
AEEVAKGGQTFTRTMNPDRVYTAVDGSSVTLHGRSLLFVRNVGHLMTIDAILDQHGNEVP
EGILDGLITSLAAIHSLNGNTSRRNSRTGSVYIVKPKMHGPEEAAFTNELFGRVEDVLNL
PRNTLKVGIMDEERRTTVNLKACIKAASERVVFINTGFLDRTGDEIHTSMEAGPMVRKAD
MKAEKWIGAYENWNVDIGLSTGLQGRAQIGKGMWAMPDLMAAMLEQKIAHPLAGANTAWV
PSPTAAALHALHYHKVDVFARQAELAKRARASVDDILTIPLAVNPNWTPEQIKNELDNNA
QGILGYVVRWIDQGVGCSKVPDINDIGLMEDRATLRISSQHIANWLRHGIVTQDQVMESL
KRMAPVVDRQNASDPLYRPLAPDFDRNIAFQAAVELVIEGTKQPNGYTEPVLHRRRREFK
AANGL