Protein Info for Pf6N2E2_4750 in Pseudomonas fluorescens FW300-N2E2

Annotation: Predicted signal-transduction protein containing cAMP-binding and CBS domains

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 646 PF00027: cNMP_binding" amino acids 52 to 139 (88 residues), 35 bits, see alignment E=2.2e-12 PF00571: CBS" amino acids 182 to 236 (55 residues), 48 bits, see alignment 2.5e-16 amino acids 250 to 297 (48 residues), 33 bits, see alignment 1.2e-11 PF03445: DUF294" amino acids 325 to 464 (140 residues), 139.1 bits, see alignment E=1.9e-44 PF10335: DUF294_C" amino acids 501 to 644 (144 residues), 151.9 bits, see alignment E=2.3e-48

Best Hits

KEGG orthology group: K07182, CBS domain-containing protein (inferred from 91% identity to pfo:Pfl01_5185)

Predicted SEED Role

"Predicted signal-transduction protein containing cAMP-binding and CBS domains" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A287 at UniProt or InterPro

Protein Sequence (646 amino acids)

>Pf6N2E2_4750 Predicted signal-transduction protein containing cAMP-binding and CBS domains (Pseudomonas fluorescens FW300-N2E2)
MVMKKADAFTQAGKTAVLQNIQGTLQFLQRFPPFNQMENAHLAYLVEQCLLRFYAPGETI
IKPADGPVEHFYIVKQGRVVGERPHTAKGGTETTFEITTGECFPLAALLGERATRTEHRA
GEDTFCLQLNKLAFIKLFALSGPFRDFALRGVSSLLDQVNQQVQQKSAQTLGTQYSLNTR
LGELAMRHPVSCSPDTPLREAVKLMHDQQVGSIVVVNEQKAPVGIFTLRDLRQVVADGAG
DFSQAIVGHMTQAPFFLSPDHSAFDAAIAMTERHIAHVCLVKDQRLCGVVSERDLFSLQR
VDLVHLARTIRNAPKVENLVALRGEIGQLVERMLAHGASSTQITHIITLLNDHTVCRVIE
LTLLDKGDPGVPFSWLCFGSEGRREQTLHTDQDNGILFEARDAAHAAQIRGLLLPIAQQI
NQSLALCGFTLCKGNIMAGNPELCLSRAEWARRFSAFIREATPENLLGSSIYFDLRVVWG
DEQGCEQLRRQILDQVADNRLFQRMMAENALRHRPPVGRFRDFVLSRKNGEKATLDLKVQ
GLTPFVDGARLLALANGIEANNTLERLRQLVDKQVIEPLDGAAYEEAYHFIQQTRMQQHQ
LQTRENLPYSNRVDPDSLNHLDRRILRESLRQAQRLQSSLTLRYQL