Protein Info for Pf6N2E2_471 in Pseudomonas fluorescens FW300-N2E2

Annotation: putative metalloprotease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 PF16485: PLN_propep" amino acids 8 to 48 (41 residues), 53.5 bits, see alignment 2.5e-18 PF01447: Peptidase_M4" amino acids 94 to 177 (84 residues), 106.1 bits, see alignment E=3.4e-34 PF02868: Peptidase_M4_C" amino acids 180 to 347 (168 residues), 186.2 bits, see alignment E=6.4e-59

Best Hits

Swiss-Prot: 63% identical to PRT1_PECCC: Extracellular metalloprotease (prt1) from Pectobacterium carotovorum subsp. carotovorum

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a3388)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZSY2 at UniProt or InterPro

Protein Sequence (353 amino acids)

>Pf6N2E2_471 putative metalloprotease (Pseudomonas fluorescens FW300-N2E2)
MFDSRPLHGFIPPYILNRIIAHGSEQQRSSALGTLTHVRSLRHNPGPPGNLPAAAKLPKP
GKAGQPQRSVHDAQNSMELPGQPVRLEGQRASGDPAVDEAYDALGATYDFFWNVLGRDSI
DNKGFALVGSVHYGQGYENAFWNGAQMVFGDGDGEIFQRFTRSLDVVAHELAHGVTESEA
GLIYANQSGALNESISDVFGVLVKQFVLEQTADQADWLIGADLLTDKINGDGLRSMSNPG
SAYDDPLLGKDPQPAHMREFVITREDNGGVHINSGIPNRAFYLVATTLGGFAWEKAGRIW
YETLCDRRLANDASFSAFARLTVEHARQRFGTREVQAVQNGWAQVGVDLTQES