Protein Info for Pf6N2E2_4657 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phosphoglycerate kinase (EC 2.7.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 96% identical to PGK_PSEPF: Phosphoglycerate kinase (pgk) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00927, phosphoglycerate kinase [EC: 2.7.2.3] (inferred from 100% identity to pba:PSEBR_a5268)

MetaCyc: 70% identical to phosphoglycerate kinase (Escherichia coli K-12 substr. MG1655)
Phosphoglycerate kinase. [EC: 2.7.2.3]

Predicted SEED Role

"Phosphoglycerate kinase (EC 2.7.2.3)" in subsystem Calvin-Benson cycle or Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 2.7.2.3)

MetaCyc Pathways

superpathway of glucose and xylose degradation (16/17 steps found)
Bifidobacterium shunt (14/15 steps found)
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
heterolactic fermentation (15/18 steps found)
superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
Calvin-Benson-Bassham cycle (11/13 steps found)
gluconeogenesis I (11/13 steps found)
Entner-Doudoroff pathway I (8/9 steps found)
formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
glycolysis II (from fructose 6-phosphate) (9/11 steps found)
glycolysis III (from glucose) (9/11 steps found)
ethene biosynthesis V (engineered) (19/25 steps found)
glycolysis IV (8/10 steps found)
glycolysis I (from glucose 6-phosphate) (10/13 steps found)
1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
homolactic fermentation (9/12 steps found)
hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
superpathway of anaerobic sucrose degradation (14/19 steps found)
oxygenic photosynthesis (12/17 steps found)
gluconeogenesis III (8/12 steps found)
glycolysis VI (from fructose) (7/11 steps found)
superpathway of hexitol degradation (bacteria) (12/18 steps found)
glycerol degradation to butanol (9/16 steps found)
superpathway of N-acetylneuraminate degradation (13/22 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A280 at UniProt or InterPro

Protein Sequence (387 amino acids)

>Pf6N2E2_4657 Phosphoglycerate kinase (EC 2.7.2.3) (Pseudomonas fluorescens FW300-N2E2)
MTVLKMSDLDLQGKRVLIREDLNVPVKDGVVTSDARILASLPTIKLALEKGAAVMVCSHL
GRPTEGEFSAENSLKPVADYLSRALGRDVPLVADYLGGVDVKAGDVVLFENVRFNKGEKK
NADELAQQYAALCDVFVMDAFGTAHRAEGSTHGVAKFAKVAAAGPLLAAELDALGKALGS
PAQPMAAIVAGSKVSTKLDVLNSLSQVCNQLIVGGGIANTFLAAAGHPVGKSLYEPDLLD
TAREIAAKVSVPLPVDVVVAKEFAESATATVKLIDDVAEDDMILDIGPQTAANFAELLKS
SKTILWNGPVGVFEFDQFGNGTKVLAQAIADSAAFSIAGGGDTLAAIDKYGVAEQISYIS
TGGGAFLEFVEGKVLPAVEVLESRAKA