Protein Info for Pf6N2E2_4632 in Pseudomonas fluorescens FW300-N2E2

Annotation: Amine oxidase [flavin-containing] (EC 1.4.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00890: FAD_binding_2
  • amino acids 30 to 69 (40 residues), 30 bits, 1.2e-10
• PF01266: DAO
  • amino acids 31 to 271 (241 residues), 54.1 bits, E=6.9e-18
• PF13450: NAD_binding_8
  • amino acids 32 to 67 (36 residues), 35.7 bits, 3.1e-12
• PF01593: Amino_oxidase
  • amino acids 37 to 452 (416 residues), 166.6 bits, E=4.5e-52

Best Hits

KEGG orthology group: K00274, monoamine oxidase [EC: 1.4.3.4] (inferred from 94% identity to pba:PSEBR_a5290)

Predicted SEED Role

"Amine oxidase [flavin-containing] (EC 1.4.3.4)" (EC 1.4.3.4)

MetaCyc Pathways

• phenylethylamine degradation I (2/2 steps found)
• putrescine degradation III (3/4 steps found)
• L-tryptophan degradation X (mammalian, via tryptamine) (2/4 steps found)
• salidroside biosynthesis (2/4 steps found)
• serotonin degradation (4/7 steps found)
• L-tryptophan degradation VI (via tryptamine) (1/3 steps found)
• noradrenaline and adrenaline degradation (8/13 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
• dopamine degradation (2/5 steps found)
• phenylethanol biosynthesis (2/5 steps found)
• aromatic biogenic amine degradation (bacteria) (4/8 steps found)
• melatonin degradation II (1/4 steps found)
• superpathway of phenylethylamine degradation (6/11 steps found)
• superpathway of melatonin degradation (1/11 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Arginine and proline metabolism
• Drug metabolism - cytochrome P450
• Glycine, serine and threonine metabolism
• Histidine metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.4

Sequence Analysis Tools

See A0A160A489 at UniProt or InterPro

Protein Sequence (587 amino acids)

>Pf6N2E2_4632 Amine oxidase [flavin-containing] (EC 1.4.3.4) (Pseudomonas fluorescens FW300-N2E2)
MSAGWLRACALVMIGLFSVSALAKDKQPTAIVIGGGLAGLTAAYELQNKGWVVTLLEAKP
SLGGRSGMATSEWIGNEKTQPVLNKYVSTFKLSTTPAPEFVRTPSYLIDGTYFTAADLAT
KQPATAEALKRYETTLDELARSIEDPQNPAANNTLHALDQITVSNWLDRLQLPATARQLV
NQEIRTRYDEPSRLSLLYFAQQNRVYRGVSDRDLRASRLLGGSPTLAQAFVKQLKTIKTN
SPVSAISQDKDGVTVKVGAVGYQADYVVLAVPLRALNKIQLTPALDAQHQGAIKGTNYGW
RDQIMLKFKTPVWDSKARMSGEIYSNTGLGMMWIEPAMKGGANVVINLSGDNARVMQAFG
DKQMVDQVLIRLHAFYPQARGAFTGYEIRRYSTDPSMGGAYLAFGPGQISKYWRLWEKPL
QRVAFAGEHTDTLYPGTLEGALRSGQRAASQVEDLAAGKSFEPAKVAPAATAAAAGAVAA
KKGNFFSNLFGGSDDEKKPEPVKAPEPVAPPPAPAPTPAPAPAPVAPPAPAPVKVEPAKK
ATAKPAAKKPAAKTPAKKAPAKAPVKKAEPVKKPAVKPTTATETKAQ