Protein Info for Pf6N2E2_4630 in Pseudomonas fluorescens FW300-N2E2
Annotation: Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 48% identical to RSME_DICD3: Ribosomal RNA small subunit methyltransferase E (rsmE) from Dickeya dadantii (strain 3937)
KEGG orthology group: K09761, ribosomal RNA small subunit methyltransferase E [EC: 2.1.1.-] (inferred from 98% identity to pba:PSEBR_a5292)Predicted SEED Role
"Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-)" in subsystem Heat shock dnaK gene cluster extended (EC 2.1.1.-)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Anthocyanin biosynthesis
- Benzoxazinone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
- Carotenoid biosynthesis - General
- Flavonoid biosynthesis
- Histidine metabolism
- Insect hormone biosynthesis
- Naphthalene and anthracene degradation
- Phenylpropanoid biosynthesis
- Porphyrin and chlorophyll metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.-
Use Curated BLAST to search for 2.1.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A087 at UniProt or InterPro
Protein Sequence (239 amino acids)
>Pf6N2E2_4630 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) (Pseudomonas fluorescens FW300-N2E2) MRLSRFFIDAPLSLGDHELPEAQAHYIGRVLRMAEGDAVQVFDGSGQEFRGTLAEVGKKR VRVQLDEQFAGQPESPLRIHLGQGLSRGERMDWAIQKATELGVSEITPIFSERCEVRLKD ERADKRLLHWRQVAISACEQCGRSSVPVIHPPLLLADWLKQTEAELKLVLHPVAEPLASH DKPSTLAFLIGPEGGLSDAEVEQAKTAGYQAARLGPRVLRTETAPVVALAVAQQLWGDF