Protein Info for Pf6N2E2_460 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glycerate kinase (EC 2.7.1.31)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00045: glycerate kinase
  • amino acids 2 to 375 (374 residues), 506.8 bits, E=1.8e-156
• PF02595: Gly_kinase
  • amino acids 3 to 373 (371 residues), 515.1 bits, E=5.1e-159

Best Hits

Swiss-Prot: 53% identical to GLXK_BACHD: Glycerate kinase (glxK) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: K00865, glycerate kinase [EC: 2.7.1.31] (inferred from 97% identity to pba:PSEBR_a3401)

MetaCyc: 80% identical to glycerate 2-kinase (Pseudomonas simiae)
GKI-RXN [EC: 2.7.1.165]

Predicted SEED Role

"Glycerate kinase (EC 2.7.1.31)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Glycine and Serine Utilization or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle (EC 2.7.1.31)

MetaCyc Pathways

• superpathway of glycol metabolism and degradation (7/7 steps found)
• glycolate and glyoxylate degradation I (4/4 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (7/9 steps found)
• superpathway of D-glucarate and D-galactarate degradation (4/5 steps found)
• D-glucarate degradation I (3/4 steps found)
• D-galactarate degradation I (3/4 steps found)
• 2-O-α-mannosyl-D-glycerate degradation (1/2 steps found)
• photorespiration II (6/10 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• 2-deoxy-D-ribose degradation II (4/8 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (16/31 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.31

Use Curated BLAST to search for 2.7.1.165 or 2.7.1.31

Sequence Analysis Tools

See A0A159ZTJ2 at UniProt or InterPro

Protein Sequence (378 amino acids)

>Pf6N2E2_460 Glycerate kinase (EC 2.7.1.31) (Pseudomonas fluorescens FW300-N2E2)
MKIVIAPDSFKDSLSAQGVADAIAQGLAEVWPDAQLIKCPMADGGEGTVESVLAACHGEW
RHTRVRGPLGVAVEARWGWLAESRTAIIEMAEASGLQLVPPGQRDACSSSTYGTGELIRA
ALDEGAGRVILAIGGSATNDAGAGAMQALGVVLLDNQGQPLPPGGLALVNLSHIDLSELD
PRLARVSFEIAADVDNPLCGPHGASVVFGPQKGASASQVQALDRALGHFAERCARALNKD
VRDEPGSGAAGGLGFAAKAFLGAQFRTGVDVVAQLTGLAEAVSGADLVITGEGRFDAQTL
RGKTPFGVARIARQHGVPVVVIAGTLGEGYQALYEHGIDAAFALASGPMTLQQACTDAPR
LLSERARDIARLWRVAHR