Protein Info for Pf6N2E2_4562 in Pseudomonas fluorescens FW300-N2E2

Annotation: L-cystine uptake protein TcyP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 transmembrane" amino acids 6 to 22 (17 residues), see Phobius details amino acids 33 to 52 (20 residues), see Phobius details amino acids 72 to 93 (22 residues), see Phobius details amino acids 104 to 127 (24 residues), see Phobius details amino acids 186 to 205 (20 residues), see Phobius details amino acids 232 to 250 (19 residues), see Phobius details amino acids 262 to 286 (25 residues), see Phobius details amino acids 297 to 317 (21 residues), see Phobius details amino acids 337 to 358 (22 residues), see Phobius details amino acids 370 to 389 (20 residues), see Phobius details amino acids 395 to 416 (22 residues), see Phobius details PF00375: SDF" amino acids 30 to 442 (413 residues), 354 bits, see alignment E=5.3e-110

Best Hits

Swiss-Prot: 79% identical to YDJN_ECOLI: L-cystine transporter YdjN (ydjN) from Escherichia coli (strain K12)

KEGG orthology group: K06956, (no description) (inferred from 98% identity to pba:PSEBR_a5357)

MetaCyc: 79% identical to cystine/sulfocysteine:cation symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-285; TRANS-RXN-286; TRANS-RXN-330; TRANS-RXN0-616

Predicted SEED Role

"L-cystine uptake protein TcyP"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3Z9 at UniProt or InterPro

Protein Sequence (463 amino acids)

>Pf6N2E2_4562 L-cystine uptake protein TcyP (Pseudomonas fluorescens FW300-N2E2)
MNLPLTLNLLVFLALLFGLAQTRHRTGSLARKVLLALVLGVAFGLALHTIYGAGNPVLKA
SIGWFDLVGNGYVQLLQMIVIPLVFASILSAVARLHNASSLGKISFLTIGTLLLTTAIAA
LIGIGLTNLFGLTAEGLVAGPQELARLQVIQSDYAGKVADLNVPQLLLSFIPQNPFADLA
RAKPTSIISVVIFAAFLGVAALQLLKDDVEKGQKVINAIDTLQSWVTRLVRLVMKLTPYG
VLALMTKVVAGSNLQDIIKLGSFVVVSYLGLGLMFVVHGLLVSAAGINPLRFFRKIWPVL
TFAFTSRSSAATIPLSIEAQTSRLGIPRTIASFSASFGATIGQNGCAGLYPAMLAVMVAP
TVGINPLDPVWIATLVAIVTLSSAGVAGVGGGATFAALIVLPAMGLPVALVALLISVEPL
IDMGRTALNVSGSITAGAITSQVMQQTDKALLNAEEHGELVHA