Protein Info for Pf6N2E2_4547 in Pseudomonas fluorescens FW300-N2E2

Annotation: Type III secretion cytoplasmic protein (YscL)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 197 PF06188: HrpE" amino acids 1 to 186 (186 residues), 105.4 bits, see alignment E=1.8e-34 TIGR02499: type III secretion apparatus protein, HrpE/YscL family" amino acids 19 to 174 (156 residues), 92.1 bits, see alignment E=2e-30

Best Hits

KEGG orthology group: K03223, type III secretion protein SctL (inferred from 90% identity to pba:PSEBR_a5372)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H8W8 at UniProt or InterPro

Protein Sequence (197 amino acids)

>Pf6N2E2_4547 Type III secretion cytoplasmic protein (YscL) (Pseudomonas fluorescens FW300-N2E2)
MLARRSLELRPDGQGLSQPYIARETLVDCARAQVILEQAQAQAAQLLGRASAEADATVLV
AQAQFWEKAETVLAAWEAQRQAMWERIETCAAHVVNEALGTLLNEVPEQARIDALVRQLA
SRQDDPVSATLRCHPEDLASLTQSLGIDDRRPWTPVADVDMERHQLKLETPGGTYLLDWP
TAVETLRLPEPQPITAS