Protein Info for Pf6N2E2_4532 in Pseudomonas fluorescens FW300-N2E2

Annotation: Flagellum-specific ATP synthase FliI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 TIGR01026: ATPase, FliI/YscN family" amino acids 22 to 446 (425 residues), 493.4 bits, see alignment E=2.8e-152 PF02874: ATP-synt_ab_N" amino acids 31 to 95 (65 residues), 29.4 bits, see alignment E=1.5e-10 PF00006: ATP-synt_ab" amino acids 157 to 366 (210 residues), 270.1 bits, see alignment E=2.1e-84 PF18269: T3SS_ATPase_C" amino acids 374 to 444 (71 residues), 72.1 bits, see alignment E=4.2e-24

Best Hits

Swiss-Prot: 70% identical to HRCN_PSESM: Type III secretion ATP synthase HrcN (hrcN) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03224, ATP synthase in type III secretion protein SctN [EC: 3.6.3.14] (inferred from 97% identity to pba:PSEBR_a5386)

Predicted SEED Role

"Flagellum-specific ATP synthase FliI" in subsystem Flagellar motility or Flagellum

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1T6 at UniProt or InterPro

Protein Sequence (451 amino acids)

>Pf6N2E2_4532 Flagellum-specific ATP synthase FliI (Pseudomonas fluorescens FW300-N2E2)
MQAAIARLEAWEAGQLAHLRRFAPVMVRGRVQRVSGILLQCRLPGAQIGDLCRVERDARE
PLLAEIVGFDQHDALLSALGGLEGVRVGAAVEPLGMPHRVCVGPQLLGQVLDGFGRPLVD
GGAGAFAGAHVPNASQVIGEAPAPTARPRIQRPLMTGVRAIDGPLTLGEGQRVGLFAGAG
CGKTTLLAEIARNVDCDVIVFGLIGERGRELREFLDHELDDELRARAVLVCATSDRSSME
RARAAFTATALAEGFRARGQRVLLLIDSLTRFARAQREIGLAAGEPLGRGGLPPSVYSLL
PRLVERAGLTNEGAITAFYTVLIEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAID
VLASLSRILANVAEAEHLRAGINLRRLLSAYEQIELMLRLGEYQAGADPLTDLAVESRGA
LDAFLRQDLREPEQLASTLAHLQQLTAYVPG