Protein Info for Pf6N2E2_4530 in Pseudomonas fluorescens FW300-N2E2

Annotation: Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 695 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 41 to 61 (21 residues), see Phobius details amino acids 73 to 93 (21 residues), see Phobius details amino acids 109 to 131 (23 residues), see Phobius details amino acids 200 to 220 (21 residues), see Phobius details amino acids 232 to 259 (28 residues), see Phobius details amino acids 285 to 301 (17 residues), see Phobius details amino acids 307 to 325 (19 residues), see Phobius details TIGR01399: type III secretion protein, HrcV family" amino acids 15 to 682 (668 residues), 830.3 bits, see alignment E=6.3e-254 PF00771: FHIPEP" amino acids 27 to 674 (648 residues), 668.5 bits, see alignment E=5.8e-205

Best Hits

Swiss-Prot: 63% identical to HRPI_ERWAM: Harpin secretion protein HrpI (hrpI) from Erwinia amylovora

KEGG orthology group: K03230, type III secretion protein SctV (inferred from 97% identity to pba:PSEBR_a5388)

Predicted SEED Role

"Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV)" in subsystem Type III secretion systems, extended

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3Y1 at UniProt or InterPro

Protein Sequence (695 amino acids)

>Pf6N2E2_4530 Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV) (Pseudomonas fluorescens FW300-N2E2)
MIALLNKVANEAVRRIEVVGAFVVLGIVFMLILPLPTSVVDALIAINICISCLLIVLSLY
LPRPLAFSSFPAVLLLTTLFRLALSVATTRLILLQGDAGHIVQAFGDFVVGGNLAVGLVI
FLILTVVNFLVITKGSERVAEVAARFTLDALPGKQMSIDSDLRANLIDVSEARRRREELN
KESQLFGAMDGAMKFVKGDAIAGLVIVVINLIGGFSVGMMQHDMGASESMHLYAVLTIGD
GLIAQIPALLISLTAGMIITRVSPQGEPAGSSVGKEIAQQLTAEPKAWVIASVGMLGFAA
LPGMPTLVFIVIALITGTAGIVQLYSRARLARQGDQPAEVVAPAQNGAEDLRSFDPTRAY
LLQFSKAMEGNAAAEEAIQAIRKKRNALVAGFGLTLPPFEIEYSALLEDDEFRFCVHEVP
MLRATFGERVAVARGPLPLEPRDGQVGSEVRDEQQWLWLTPDDPLLADERVEAVSAAELL
VERMSRAMFATGPQFLGLQESKSILSWLEAGQPELVQELQRTMPLARFAAVLQRLAGEGV
PLRAVRLIAESLIEHGQHERDVGALTDYARIALKSQIYHQYRDEDGLHVWLLTPETEGLL
RDALRQTQTETFFALESLHTRALVMQLREGFPLRAKRVAVLLVAQDLRAPLRDLLRDEFN
HVPVLAFSELHSNAQVTVLGRFDLEQEKLRMEDAA