Protein Info for Pf6N2E2_4504 in Pseudomonas fluorescens FW300-N2E2

Annotation: Alkanesulfonate monooxygenase (EC 1.14.14.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 TIGR03565: alkanesulfonate monooxygenase, FMNH(2)-dependent" amino acids 4 to 349 (346 residues), 633.9 bits, see alignment E=3e-195 PF00296: Bac_luciferase" amino acids 4 to 326 (323 residues), 275.6 bits, see alignment E=3.1e-86

Best Hits

Swiss-Prot: 92% identical to SSUD_BUTSP: Alkanesulfonate monooxygenase (ssuD) from Buttiauxella sp. (strain PNBS)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 99% identity to pba:PSEBR_a5411)

MetaCyc: 91% identical to alkanesulfonate monooxygenase subunit (Pseudomonas putida DS1)
RXN-18086 [EC: 1.14.14.34]

Predicted SEED Role

"Alkanesulfonate monooxygenase (EC 1.14.14.5)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.14.14.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.14.14.5

Use Curated BLAST to search for 1.14.14.34 or 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A414 at UniProt or InterPro

Protein Sequence (382 amino acids)

>Pf6N2E2_4504 Alkanesulfonate monooxygenase (EC 1.14.14.5) (Pseudomonas fluorescens FW300-N2E2)
MSLNIFWFLPTHGDGHYLGTAEGARAVDHGYLQQIAQAADRLGFGGVLIPTGRSCEDSWL
VAASLIPVTQRLKFLVALRPGIISPTVAARQAATLDRLSGGRALFNLVTGGDPDELAGDG
LFLDHEQRYQASVEFTRIWRRVLEGETVDYDGEHISVKGAKLLYPPIQQPRPPLYFGGSS
EAAQDLAAEQVEMVLTWGEPPAAVAEKIQQVRAKAAKLGRTVRFGIRLHVIVRETNAEAW
QAADRLISHLDDETIARAQASLARFDSVGQQRMAALHGGRRDKLEVSPNLWAGVGLVRGG
AGTALVGDGPTVAARVKEYAELGIDTFIFSGYPHLEESYRVAELLFPHLDVERPELPKSQ
GYVSPFGEMVANDILPKAASQS