Protein Info for Pf6N2E2_4491 in Pseudomonas fluorescens FW300-N2E2

Annotation: Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 PF05157: MshEN" amino acids 84 to 169 (86 residues), 42 bits, see alignment E=7.4e-15 PF00437: T2SSE" amino acids 203 to 586 (384 residues), 433.8 bits, see alignment E=4.4e-134

Best Hits

KEGG orthology group: K02454, general secretion pathway protein E (inferred from 100% identity to pba:PSEBR_a5422)

Predicted SEED Role

"Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H8W2 at UniProt or InterPro

Protein Sequence (594 amino acids)

>Pf6N2E2_4491 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB (Pseudomonas fluorescens FW300-N2E2)
MSTKHATQDRWLDLNDLLRQLVTQGLISQDSAETALNARRRHSANSQLHPLEFIANQHLD
DLSRPGKRLDLENLTLWLAQQADQPYMRIDPLKINVAAVTPLMSYAFAQRHKILAVAVDR
DAVTVASAQPYVNSWEADLTHVLKLPIKRVVANPVDIQRLSVEFYRLAKSVSGATTVDQQ
PSNLSNFEQLLNLGASDQEPDANDAHIVNIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHTVYQFPPQVTMAIVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGGEVELRLSTLP
TAFGEKMVMRIFDPEVLLKDFDQLGFSADDLRRWQDMTRQPNGIILVTGPTGSGKTTTLY
TTLKKLATPEVNLCTIEDPIEMVEASFNQMQVQHNIDLSFAAGVRALMRQDPDIIMIGEI
RDLETAEMAIQAALTGHLVLSTLHTNDAPSAISRLLELGVPHYLIKATVLGVMAQRLVRT
LCPQCKAPLTLDEDDWQTLTRPWQAPLPSNAQQAVGCLECRDTGYRGRAGVYEIMQLTDS
VKALIHPDTDLLAVRRQAFKEGMRSLRLSGAQKVAAGLTTVEEVLRVTPQSEQK