Protein Info for Pf6N2E2_446 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glutathione reductase (EC 1.8.1.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 TIGR01424: glutathione-disulfide reductase" amino acids 3 to 447 (445 residues), 608.9 bits, see alignment E=2.3e-187 PF07992: Pyr_redox_2" amino acids 6 to 317 (312 residues), 229.6 bits, see alignment E=1.4e-71 PF13738: Pyr_redox_3" amino acids 121 to 294 (174 residues), 54.9 bits, see alignment E=2.1e-18 PF00070: Pyr_redox" amino acids 168 to 247 (80 residues), 58.9 bits, see alignment E=1.5e-19 PF02852: Pyr_redox_dim" amino acids 338 to 446 (109 residues), 128.2 bits, see alignment E=4e-41

Best Hits

Swiss-Prot: 77% identical to GSHR_PSEAE: Glutathione reductase (gor) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00383, glutathione reductase (NADPH) [EC: 1.8.1.7] (inferred from 97% identity to pba:PSEBR_a3415)

MetaCyc: 45% identical to glutathione reductase (NADPH) (Escherichia coli K-12 substr. MG1655)
Glutathione-disulfide reductase. [EC: 1.8.1.7]

Predicted SEED Role

"Glutathione reductase (EC 1.8.1.7)" in subsystem Glutathione: Redox cycle (EC 1.8.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZRN1 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Pf6N2E2_446 Glutathione reductase (EC 1.8.1.7) (Pseudomonas fluorescens FW300-N2E2)
MAYDFDLYVIGAGSGGVRAARFAAGFGAKVAVAESRYLGGTCVNVGCVPKKLLVYGAHYA
EDFEQASAYGWTAGEPSFDWATLIANKDREINRLNGIYRSLLVNSGVVLHEGHARLTGPN
EVEINGQRYTAKHILIATGGWPVIPDIPGREHAISSNEAFFLKELPKRVIVVGGGYIAVE
FAGIFHGMGAQTSLLYRGGLFLRGFDGAVRKHLAEELTRRGLDLQFDADIKCIEKLDDGS
LRVELKNGQTLLTDCVFYATGRRPMLDNLGLENTAVTVDEKGFVQVNEKYETTEPSILAI
GDVIGRVQLTPVALAEGMAVARRLFKPEQYRLVDYRMIPTAVFSLPNIGTVGLTEEQARE
EGHEVQIFESRFRPMKLSLTECQERTLMKLVVDARTDKVLGCHMVGPDAGEIVQGLAIAL
KAGATKRDFDETIGVHPTAAEEFVTMRTPVAS