Protein Info for Pf6N2E2_4453 in Pseudomonas fluorescens FW300-N2E2

Annotation: Polyphosphate kinase (EC 2.7.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 transmembrane" amino acids 466 to 482 (17 residues), see Phobius details PF13089: PP_kinase_N" amino acids 55 to 160 (106 residues), 123.3 bits, see alignment E=1.4e-39 TIGR03705: polyphosphate kinase 1" amino acids 55 to 728 (674 residues), 938.4 bits, see alignment E=1.2e-286 PF02503: PP_kinase" amino acids 171 to 352 (182 residues), 207.9 bits, see alignment E=2.7e-65 PF17941: PP_kinase_C_1" amino acids 379 to 542 (164 residues), 233.3 bits, see alignment E=3.1e-73 PF13090: PP_kinase_C" amino acids 551 to 718 (168 residues), 217.8 bits, see alignment E=1.8e-68

Best Hits

Swiss-Prot: 92% identical to PPK1_PSESM: Polyphosphate kinase (ppk) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to pba:PSEBR_a5456)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1N1 at UniProt or InterPro

Protein Sequence (738 amino acids)

>Pf6N2E2_4453 Polyphosphate kinase (EC 2.7.4.1) (Pseudomonas fluorescens FW300-N2E2)
MNTEGLSEVAVKDAQPVVEQIAETPPTLEPAAPAVVEPAPAPAIVIPNLDDSSLYIHREL
SQLQFNIRVLEQALDESYPLLERLKFLLIFSSNLDEFFEIRVAGLKKQITFAREQAGADG
LQPHQALARISELVHGHVDRQYAILNDILLPELEKHQVRFIRRRYWNTKIKTWVRRYFRD
EIAPIITPIGLDPTHPFPLLVNKSLNFIVELEGIDAFGRDSGLAIIPAPRLLPRIIRVPE
DVGGPGDNYVFLSSMIHAHADDLFQGMKVKGCYQFRLTRNADLSVDTEDVEDLARALRGE
LFSRRYGDAVRLEVADTCPKHLSDYLLKQFNLHETELYQVNGPVNLTRLFSITGLDSHPE
LQYLPFTPQIPKLLQNSENIFSVISKQDILLLHPFESFTPVVDLLRQAAKDPHVLAVRQT
LYRSGANSEIVDALVDAARNGKEVTAVIELRARFDEESNLQLASRLQAAGAVVIYGVVGF
KTHAKMMLILRREAGEIVRYAHLGTGNYHAANARLYTDYSLLTSDDALCEDVGKLFSQLI
GMGKTLRMKKLLHAPFTLKKGMLDMIARETQFALDGKPAHIIAKFNSLTDPKIIRALYKA
SQSGVRIDLVVRGMCCLRPGIAGVSHNIHVRSIIGRFLEHTRVFYFLNGGEEQMFLSSAD
WMERNLDKRVETCFPVEGKKLILRVKKELESYLTDNTHSWSLQSDGRYIRNTPTGNQNPR
SAQATLLERLSSPVLAVR