Protein Info for Pf6N2E2_4450 in Pseudomonas fluorescens FW300-N2E2

Annotation: Sterol desaturase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 45 to 65 (21 residues), see Phobius details amino acids 77 to 94 (18 residues), see Phobius details amino acids 134 to 160 (27 residues), see Phobius details amino acids 301 to 319 (19 residues), see Phobius details amino acids 325 to 347 (23 residues), see Phobius details amino acids 359 to 378 (20 residues), see Phobius details amino acids 384 to 402 (19 residues), see Phobius details PF04116: FA_hydroxylase" amino acids 80 to 213 (134 residues), 109.1 bits, see alignment E=1.1e-35

Best Hits

KEGG orthology group: None (inferred from 95% identity to pba:PSEBR_a5459)

Predicted SEED Role

"Sterol desaturase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A2V4 at UniProt or InterPro

Protein Sequence (411 amino acids)

>Pf6N2E2_4450 Sterol desaturase (Pseudomonas fluorescens FW300-N2E2)
MNFILYAVPFFFVLIAVELLADRWRGVSHYRVADAINSLSTGVLSTTTGLLTKGVGLVTY
AFALKHLAVIELPADRAWTWVFAFVLYDFCYYWLHRMGHERNILWAAHSVHHQSEDYNLS
TALRQTSTGFLLSWIFYVPLAVLGVPLVVFVSVAALNLLYQFWVHTQHIPKLGWLEWCFV
TPSNHRAHHAQNPLYMDRNYGGVFIIWDRLFGTFQEEDVNEPVIFGVTTPLESWNPLWAN
LQFYAQLWADARRAGRWWDKLRIWFMPTGWRPADVAARYPLNKSDLSQFRKFDVPLDVRQ
QWYVGLQFCAYIALGSYLMNLEASLPVAALMLGWGAVAFGLFVLGVALENRPWALRLELL
RLASNLPLVWLAPLAGLWPANPVAWAGLLSYSLLSGIGLYCCRARFTRLAS