Protein Info for Pf6N2E2_4383 in Pseudomonas fluorescens FW300-N2E2
Updated annotation (from data): gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.99); 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54)
Rationale: Important on putrescine; this is the dehydrogenase reaction in the gamma-glutamyl-putrescine pathway. Similar to the 4-guanidinobutyraldehyde dehydrogenase kauB from P. putida, which is involved in arginine catabolism, and 99% id. to AO356_12580, which has a phenotype on arginine. So Pf6N2E2_4383 probably has both activities.
Original annotation: Aldehyde dehydrogenase (EC 1.2.1.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 44% identical to AL1A2_MOUSE: Retinal dehydrogenase 2 (Aldh1a2) from Mus musculus
KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a5516)MetaCyc: 88% identical to 4-guanidinobutyraldehyde dehydrogenase (Pseudomonas putida)
Gamma-guanidinobutyraldehyde dehydrogenase. [EC: 1.2.1.54]
Predicted SEED Role
"Aldehyde dehydrogenase (EC 1.2.1.3)" in subsystem Entner-Doudoroff Pathway or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.1.3)
MetaCyc Pathways
- superpathway of glycol metabolism and degradation (7/7 steps found)
- superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
- superpathway of ornithine degradation (7/8 steps found)
- putrescine degradation II (4/4 steps found)
- superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
- ethanol degradation II (3/3 steps found)
- ethanol degradation IV (3/3 steps found)
- ethylene glycol degradation (2/2 steps found)
- putrescine degradation V (2/2 steps found)
- Entner-Doudoroff pathway II (non-phosphorylative) (7/9 steps found)
- mitochondrial NADPH production (yeast) (4/5 steps found)
- octane oxidation (4/5 steps found)
- L-arginine degradation IX (arginine:pyruvate transaminase pathway) (3/4 steps found)
- phytol degradation (3/4 steps found)
- putrescine degradation III (3/4 steps found)
- ethanol degradation III (2/3 steps found)
- hypotaurine degradation (2/3 steps found)
- putrescine degradation IV (2/3 steps found)
- putrescine degradation I (1/2 steps found)
- D-arabinose degradation II (2/4 steps found)
- L-arginine degradation VIII (arginine oxidase pathway) (2/4 steps found)
- L-tryptophan degradation X (mammalian, via tryptamine) (2/4 steps found)
- fatty acid α-oxidation I (plants) (2/4 steps found)
- serotonin degradation (4/7 steps found)
- histamine degradation (1/3 steps found)
- 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
- noradrenaline and adrenaline degradation (8/13 steps found)
- dopamine degradation (2/5 steps found)
- aromatic biogenic amine degradation (bacteria) (4/8 steps found)
- ceramide degradation by α-oxidation (2/7 steps found)
- sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
- alkane oxidation (1/6 steps found)
- limonene degradation IV (anaerobic) (1/7 steps found)
- ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
- superpathway of pentose and pentitol degradation (15/42 steps found)
KEGG Metabolic Maps
- 1,2-Dichloroethane degradation
- 3-Chloroacrylic acid degradation
- Arginine and proline metabolism
- Ascorbate and aldarate metabolism
- Butanoate metabolism
- Fatty acid metabolism
- Glycerolipid metabolism
- Glycolysis / Gluconeogenesis
- Histidine metabolism
- Limonene and pinene degradation
- Lysine degradation
- Propanoate metabolism
- Pyruvate metabolism
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.3
Use Curated BLAST to search for 1.2.1.3 or 1.2.1.54 or 1.2.1.99
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZZQ3 at UniProt or InterPro
Protein Sequence (497 amino acids)
>Pf6N2E2_4383 gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.99); 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (Pseudomonas fluorescens FW300-N2E2) MTTLTRADWEQRARDLKIEGRAFINGEYTDAVSGETFDCLSPVDGRLLGKIASCDVADAQ RAVENARATFSSGVWSRLAPSKRKATMIRFAGLLKQHAEELALLETLDMGKPISDSLNID VPGAAQALSWSGEAIDKLYDEVAATPHDQLGLVTREPVGVVGAIVPWNFPLMMACWKLGP ALSTGNSVVLKPSEKSPLTALRIAALAIEAGIPKGVLNVLPGYGHTVGKALALHMDVDTL VFTGSTKIAKQLMIYSGESNMKRIWLEAGGKSPNIVFADAPDLQAAAESAASAIAFNQGE VCTAGSRLLVERSIKDTFLPLVIEALKGWKPGNPLDPATNVGALVDTQQMNTVLSYIEAG HSDGAKLVAGGKRILEETGGTYVEPTIFDGVSNAMKIAQEEIFGPVLSVIAFDTAEQAIE IANDTPYGLAAAVWTKDISKAHLTAKALRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKS LHAFDKYTELKSTWIKL