Protein Info for Pf6N2E2_4376 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR00521: phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase" amino acids 6 to 395 (390 residues), 470.4 bits, see alignment E=2.4e-145 PF02441: Flavoprotein" amino acids 7 to 177 (171 residues), 113.4 bits, see alignment E=8.4e-37 PF04127: DFP" amino acids 186 to 370 (185 residues), 236.7 bits, see alignment E=1.8e-74

Best Hits

Swiss-Prot: 62% identical to COABC_VIBPA: Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K13038, phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase [EC: 4.1.1.36 6.3.2.5] (inferred from 100% identity to pba:PSEBR_a5521)

Predicted SEED Role

"Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)" in subsystem Coenzyme A Biosynthesis (EC 4.1.1.36, EC 6.3.2.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.36 or 6.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WV80 at UniProt or InterPro

Protein Sequence (402 amino acids)

>Pf6N2E2_4376 Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5) (Pseudomonas fluorescens FW300-N2E2)
MQRLYRKRIVLGVGGGIAAYKSAELVRRLIDQGAEVRVVMTRGGSEFITPLTMQALSGHP
VHLDLLDPAAEAAMGHIELAKWADLVLIAPATADLIARLAQGIADDLLTTLVLATDAVVA
VAPAMNQAMWRDPATQANLQLLESRALKVFGPASGSQACGDVGMGRMLEATDLAQCAADC
FQRQALTGKHVLITAGPTQENIDPVRYITNHSSGKMGFALAEAAVEAGARVTLITGPVHL
PTPDRVTRIDVVSARDMLAACEAAIPCDLFIASAAVADYRPEVVAPQKLKKDPTSGDGLL
LQMVRNPDILASIATRPDRPFSVGFAAETEHLLDYAARKLKDKNLDLIVANDVANPSIGF
NSEENACSVIDRQLHATLFAQTSKSKIARQLITFIAERLNQV