Protein Info for Pf6N2E2_4374 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02878: PGM_PMM_I
  • amino acids 15 to 144 (130 residues), 124.2 bits, E=6.3e-40
• PF02879: PGM_PMM_II
  • amino acids 160 to 257 (98 residues), 100.6 bits, E=1.3e-32
• PF02880: PGM_PMM_III
  • amino acids 262 to 368 (107 residues), 85.2 bits, E=7.3e-28
• PF00408: PGM_PMM_IV
  • amino acids 377 to 454 (78 residues), 54.5 bits, E=2e-18

Best Hits

Swiss-Prot: 82% identical to ALGC_PSESM: Phosphomannomutase/phosphoglucomutase (algC) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a5523)

MetaCyc: 78% identical to phosphomannomutase (Pseudomonas aeruginosa)
Phosphomannomutase. [EC: 5.4.2.8]; Phosphoglucomutase. [EC: 5.4.2.8, 5.4.2.2]

Predicted SEED Role

"Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2)" in subsystem Alginate metabolism or Mannose Metabolism (EC 5.4.2.2, EC 5.4.2.8)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• GDP-mannose biosynthesis (4/4 steps found)
• colanic acid building blocks biosynthesis (9/11 steps found)
• glycogen degradation II (5/6 steps found)
• UDP-α-D-glucose biosynthesis (2/2 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• glucose and glucose-1-phosphate degradation (4/5 steps found)
• sucrose degradation II (sucrose synthase) (4/5 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycogen degradation I (6/8 steps found)
• sucrose biosynthesis II (6/8 steps found)
• glycogen biosynthesis I (from ADP-D-Glucose) (3/4 steps found)
• starch degradation V (3/4 steps found)
• sucrose degradation IV (sucrose phosphorylase) (3/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• GDP-α-D-glucose biosynthesis (2/3 steps found)
• trehalose degradation V (2/3 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• CDP-6-deoxy-D-gulose biosynthesis (3/5 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• starch degradation III (2/4 steps found)
• glucosylglycerol biosynthesis (2/5 steps found)
• starch biosynthesis (5/10 steps found)
• glycogen biosynthesis III (from α-maltose 1-phosphate) (3/8 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (7/14 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
• streptomycin biosynthesis (3/18 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Pentose phosphate pathway
• Starch and sucrose metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 5.4.2.2

Use Curated BLAST to search for 5.4.2.2 or 5.4.2.8

Sequence Analysis Tools

See A0A161H8U9 at UniProt or InterPro

Protein Sequence (465 amino acids)

>Pf6N2E2_4374 Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2) (Pseudomonas fluorescens FW300-N2E2)
MNTPAAIAPIFPDSIFRAYDIRGVVPETLTAETAYWIGRAIGSQSLAQGEPNVCVGRDGR
LSGPELVEQLIKGVADSGCHVSDVGLVPTPALYYAANVLAGKSGVMLTGSHNPSNYNGFK
IVIAGDTLANEQIQALHTRLKTNDLSSGQGSITKVDILERYNDEIVKDVKLARRLKVVVD
CGNGAAGVIAPQLIEALNCEVIPLFCDVDGNFPNHHPDPGKLENLEDLIAKVKETNADLG
LAFDGDGDRVGVVTNTGSVVFPDRLLMLFAKDVVARNPNAEIIFDVKCTRRLVPLIKEYG
GRPLMWKTGHSLIKKKMKQSGALLAGEMSGHIFFKERWFGFDDGIYSAARLLEILSKEKS
TAEELFATFPNDISTPEINIHVTEESKFSIIDALHDAQWGEGAELTTIDGVRVDYPHGWG
LVRASNTTPVLVLRFEADNEAELQRIKDVFHTQLKRVAPDLQLPF