Protein Info for Pf6N2E2_436 in Pseudomonas fluorescens FW300-N2E2

Annotation: ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 PF02805: Ada_Zn_binding" amino acids 15 to 75 (61 residues), 97.7 bits, see alignment E=7.6e-32 PF00165: HTH_AraC" amino acids 106 to 133 (28 residues), 33 bits, see alignment (E = 1.3e-11) PF12833: HTH_18" amino acids 109 to 186 (78 residues), 70 bits, see alignment E=4.5e-23 PF02870: Methyltransf_1N" amino acids 194 to 270 (77 residues), 22.6 bits, see alignment E=3.3e-08 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 273 to 352 (80 residues), 116.7 bits, see alignment E=1.8e-38 PF01035: DNA_binding_1" amino acids 274 to 353 (80 residues), 121.1 bits, see alignment E=3.5e-39

Best Hits

KEGG orthology group: K10778, AraC family transcriptional regulator, regulatory protein of adaptative response / methylated-DNA-[protein]-cysteine methyltransferase [EC: 2.1.1.63] (inferred from 98% identity to pba:PSEBR_a3425)

Predicted SEED Role

"ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.63

Use Curated BLAST to search for 2.1.1.63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YUB9 at UniProt or InterPro

Protein Sequence (357 amino acids)

>Pf6N2E2_436 ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (Pseudomonas fluorescens FW300-N2E2)
MNSTSNHLAPELDPRWAAVLARDPRADGTFVYGVKTTGIYCHPSSLSRLPNPRNVEFFDT
PEQAQAAGYRPSKRVARDQTQIAAQQAARVAAACRQIEAAEQLPGLNELAESAGLSPFHF
HRVFKAVTGLTPKGYAAAHRSRKVRERLADGGSITEALYDAGFNSNSRFYEAADKVLGMK
PADYRAAGQNTDIRFAVGQCSLGAILVAQSERGVCAILLGDDPDALVRDLQDKFRRANLI
GADREFEQLIAQVVGFIETPALGLDLPLDLRGTAFQERVWQALRDIPAGSTASYAEIAQR
IGMPKAVRAVAQACGANSLAVAIPCHRVVRSDGNLSGYRWGVERKRQLLALERSSGD