Protein Info for Pf6N2E2_4344 in Pseudomonas fluorescens FW300-N2E2

Annotation: Serine/threonine protein kinase (EC 2.7.11.1) PpkA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1032 transmembrane" amino acids 341 to 363 (23 residues), see Phobius details PF00069: Pkinase" amino acids 12 to 253 (242 residues), 160.5 bits, see alignment E=1.1e-50 PF07714: PK_Tyr_Ser-Thr" amino acids 16 to 252 (237 residues), 113.5 bits, see alignment E=2.2e-36

Best Hits

KEGG orthology group: K11912, serine/threonine-protein kinase PpkA [EC: 2.7.11.1] (inferred from 99% identity to pba:PSEBR_a5549)

Predicted SEED Role

"Serine/threonine protein kinase (EC 2.7.11.1) PpkA" (EC 2.7.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.11.1

Use Curated BLAST to search for 2.7.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3R8 at UniProt or InterPro

Protein Sequence (1032 amino acids)

>Pf6N2E2_4344 Serine/threonine protein kinase (EC 2.7.11.1) PpkA (Pseudomonas fluorescens FW300-N2E2)
MSLTLNIVIPGYDIEGPIGEGAMASVYLATQRSLERKVALKVMAAALAADPTFCERFLRE
GKTLARLSHPHTVTIHDIGNVGELYYMAMEYLPNGTLKERIAAGLTPEQGLTYIRQIASA
LGYAHGLGLVHRDVKPANILFRADGTAVLSDFGIAKSLDDRTQFTQAGFAVGTPSYMSPE
QARGQDIDGRADLYALGVVLYEILVGKLPYTGNDALSTALAHLTEPLPELPVHHGRYQDV
LRKLLAKDPAERFPDAAALLRALDNLPDESAEATLIRPLSLQLPETPKPVGDLAGLTPMS
IDIPSGPAYSPPPPQSQPKPVAPPIKPTVQSSVSEQRKGPVFALAAVAVAVALALGGAGY
WWLSGDDGKAVKTPVATTTSVKPPEVKPPQAPEVPAVKPPVVPAAQPPVATEADGGQRPL
LMAGKKTLFQRVLSKPGAKLADAPGAAPGKALPAFSVLYVYQRKDVDGGSWVRVGAATDG
RSDGWLPAAQVSDWKQSLVLKFTERSGRAPVMFLRQPGDVEKLLANPSASKNVLLKAQQS
PQEDQQVLALEPAASAVPQSQFYLLPIFDSRESLDENGQPVQLLNVASIDPGNTPKASSN
TPITTANADAFRTAVVLVVDTTVSMQPYIDQVRDVVHELQTRIAERGELDSVSFGMVGFR
SSIKKTPGLEYVAKTLITLEQGRDPQRFMELARQVKASTVSSHSFNEDAFAGVMEAVEGM
DWSGYGGRLILLVTDAGALRKNDPFAATQMNEAEVRQAALGKQIKIYALHLLSDAGKKTH
AGAQSQYRTLTADANPQIGDLYIPVPGADVRKFGERVDEIGSVFADLVHQVRSNKAQSVP
LLSAAPSLADKSAAVGYAMHMDFLGRKSASQAPQLVSAWTADRDLTNPALPAFQVCVMLT
KLQLNDLQQSLKLIVDAARKTQSSPKDFFQEIASASAYMSRDPSALRKGGNLADGGILGE
YLEGLPYRSKSLNMTQDLWLSLSVAEQEDFIDELDSKIRLYETFHNDLANWVRFGDAEPG
DALYRVPLSTLP