Protein Info for Pf6N2E2_43 in Pseudomonas fluorescens FW300-N2E2

Annotation: Histidine permease YuiF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 transmembrane" amino acids 7 to 36 (30 residues), see Phobius details amino acids 49 to 73 (25 residues), see Phobius details amino acids 102 to 114 (13 residues), see Phobius details amino acids 118 to 137 (20 residues), see Phobius details amino acids 146 to 171 (26 residues), see Phobius details amino acids 191 to 213 (23 residues), see Phobius details amino acids 234 to 253 (20 residues), see Phobius details amino acids 259 to 278 (20 residues), see Phobius details amino acids 291 to 310 (20 residues), see Phobius details amino acids 331 to 350 (20 residues), see Phobius details amino acids 361 to 386 (26 residues), see Phobius details amino acids 421 to 438 (18 residues), see Phobius details PF13726: Na_H_antiport_2" amino acids 3 to 87 (85 residues), 103.9 bits, see alignment E=6.5e-34 PF03553: Na_H_antiporter" amino acids 150 to 433 (284 residues), 274.6 bits, see alignment E=1.6e-85 PF06808: DctM" amino acids 185 to 389 (205 residues), 32.3 bits, see alignment E=7.6e-12

Best Hits

Swiss-Prot: 60% identical to Y2115_VIBPA: Uncharacterized membrane protein VP2115 (VP2115) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K07084, (no description) (inferred from 99% identity to pba:PSEBR_a3888)

Predicted SEED Role

"Histidine permease YuiF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YHJ3 at UniProt or InterPro

Protein Sequence (439 amino acids)

>Pf6N2E2_43 Histidine permease YuiF (Pseudomonas fluorescens FW300-N2E2)
MINAVIAAVGVMLVLSLARVHVVIALIIGALVGGLTGGLGIEATLKAFNAGLGGGATVAL
SYALLGAFAVAIAKSGMAHALADKALALVDRQDTAGGRGVKWVLIGLLGTVAVASQNILP
IHIAFIPLLVPPLLYVLTKLQLDRRLIACVMTFGLITPYMFLPVGFGNIFLNEILLANIA
RSGVDVSGINVTHAMGIPALGMVFGLLLSLFSYRKKRVYDLKKIARVEQVAVRYNPLSLM
VAGFAVAAAFAVQLLVDSMIIGALVGFLIFSLSGVVRWRETDDLFTEGMKMMAMIGFIMI
AASGFAEVMKATGEVQSLVESSAAWIGHNKGIGALMMLLVGLLVTMGIGSSFSTVPILAT
IFVPLCLQLGFSPLAIVCIVGTAGALGDAGSPASDSTLGPTSGLNIDGQHHHIWDTVVPT
FIHYNLPLLVFGWVAAMVL