Protein Info for Pf6N2E2_4278 in Pseudomonas fluorescens FW300-N2E2
Annotation: Pyruvate carboxyl transferase subunit A (EC 6.4.1.1)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to 2OCS_HYDTT: 2-oxoglutarate carboxylase small subunit (cfiB) from Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6)
KEGG orthology group: None (inferred from 100% identity to pba:PSEBR_a5593)MetaCyc: 53% identical to pyruvate carboxylase subunit A (Methanothermobacter thermautotrophicus Delta H)
Pyruvate carboxylase. [EC: 6.4.1.1]
Predicted SEED Role
No annotation
MetaCyc Pathways
- gluconeogenesis III (8/12 steps found)
- reductive TCA cycle I (6/11 steps found)
- incomplete reductive TCA cycle (3/7 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (4/13 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.4.1.1
Use Curated BLAST to search for 6.4.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A3F7 at UniProt or InterPro
Protein Sequence (471 amino acids)
>Pf6N2E2_4278 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) (Pseudomonas fluorescens FW300-N2E2) LITKILIANRGEIAVRIVRACAEMGIRSVAVYSDADRHALHVKRADEAHSIGADPLAGYL NPRKLVNLAVETGCDALHPGYGFLSENAELADICAERGIKFIGPSAEVIRRMGDKTEARR SMIKAGVPVTPGTEGNVSGIEEALTEGDRIGYPVMLKATSGGGGRGIRRCNSREELEQAF PRVISEATKAFGSAEVFLEKCIVNPKHIEAQILGDSFGNVVHLFERDCSIQRRNQKLIEI APSPQLTPEQRAYIGDLSVRAAKAVGYENAGTVEFLLAEGEVYFMEMNTRVQVEHTITEE ITGIDIVREQIRIASGLPLSVKQEDIVHRGFALQFRINAEDPKNNFLPSFGKITRYYAPG GPGVRTDTAIYTGYTIPPFYDSMCLKLVVWALTWEEAMDRGLRALDDMRLQGVKTTAAYY QEILRNPEFRSGQFNTSFVESHPELTNYSIKRKPEELALAIAAAIAAHAGL