Protein Info for Pf6N2E2_4268 in Pseudomonas fluorescens FW300-N2E2

Annotation: Pyridoxal kinase (EC 2.7.1.35)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00687: pyridoxal kinase
  • amino acids 6 to 285 (280 residues), 377.2 bits, E=2.8e-117
• PF08543: Phos_pyr_kin
  • amino acids 71 to 257 (187 residues), 46.1 bits, E=4.3e-16
• PF00294: PfkB
  • amino acids 134 to 264 (131 residues), 34.2 bits, E=1.9e-12

Best Hits

Swiss-Prot: 90% identical to PDXY_PSEPF: Pyridoxal kinase PdxY (pdxY) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00868, pyridoxine kinase [EC: 2.7.1.35] (inferred from 98% identity to pba:PSEBR_a5602)

MetaCyc: 58% identical to pyridoxal kinase 2 (Escherichia coli K-12 substr. MG1655)
Pyridoxal kinase. [EC: 2.7.1.35]

Predicted SEED Role

"Pyridoxal kinase (EC 2.7.1.35)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.7.1.35)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
• pyridoxal 5'-phosphate salvage I (6/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (6/9 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (5/8 steps found)

KEGG Metabolic Maps

• Vitamin B6 metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.35

Sequence Analysis Tools

See A0A160A3E6 at UniProt or InterPro

Protein Sequence (290 amino acids)

>Pf6N2E2_4268 Pyridoxal kinase (EC 2.7.1.35) (Pseudomonas fluorescens FW300-N2E2)
MKRTPHLLAIQSHVVFGHAGNSAAVFPMQRVGVNVWPLNTVQFSNHTQYGQWTGEVLAPE
QIPALVEGIAAIGELGHCDAVLSGYLGSAAQGRAILTGVARIKAANPKALYLCDPVMGHP
EKGCIVAPEVSEFLLEEAAAVADLMCPNQLELDSFSGRKAQSLLDCLAMARALLARGPKA
VLVKHLAYPGKPDDSFEMLLVTADGNWHLRRPLLAFPRQPVGVGDLTSGLFLARILLGDS
LVAALEFTAAAVHEVLLETQACTSYELELVRAQDRIAHPRVRFEAVPISL