Protein Info for Pf6N2E2_4255 in Pseudomonas fluorescens FW300-N2E2

Annotation: Probable cadmium-transporting ATPase (EC 3.6.3.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details amino acids 51 to 73 (23 residues), see Phobius details amino acids 85 to 111 (27 residues), see Phobius details amino acids 250 to 268 (19 residues), see Phobius details amino acids 274 to 302 (29 residues), see Phobius details amino acids 574 to 595 (22 residues), see Phobius details amino acids 601 to 622 (22 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 83 to 622 (540 residues), 516 bits, see alignment E=2.4e-158 TIGR01512: cadmium-translocating P-type ATPase" amino acids 83 to 624 (542 residues), 478.7 bits, see alignment E=3.2e-147 TIGR01494: HAD ATPase, P-type, family IC" amino acids 107 to 594 (488 residues), 195.5 bits, see alignment E=1.8e-61 PF00122: E1-E2_ATPase" amino acids 140 to 305 (166 residues), 172.7 bits, see alignment E=5.6e-55 PF00702: Hydrolase" amino acids 324 to 528 (205 residues), 59.3 bits, see alignment E=7.1e-20

Best Hits

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a5615)

Predicted SEED Role

"Probable cadmium-transporting ATPase (EC 3.6.3.3)" in subsystem Cobalt-zinc-cadmium resistance (EC 3.6.3.3)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3

Use Curated BLAST to search for 3.6.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1D2 at UniProt or InterPro

Protein Sequence (639 amino acids)

>Pf6N2E2_4255 Probable cadmium-transporting ATPase (EC 3.6.3.3) (Pseudomonas fluorescens FW300-N2E2)
MTAQTSAAPMLSSTEQRIAARQLTLAMLALGLLALGLVWRGWSPEQSGVSQLLLGFASLL
VAVPVMRSAWYSLRYPSLHGITDQLIALAMLGAWATGDLLTAALLPIIMIFGHVLEERSV
IGSQEAIHALGQLTRSQGRKVQADGSIIDVDNATLQPGDVVEVRAGDRVPADGRVLSGQA
SLDTAPITGESVPLEAVVGTEVFGGAINLDGLLRLEVTRTGEESTLGKVIALMQNAERSK
PPITRLLERYAGSYLVLVLLLAAVTWFVTNDAQAMLAVLVAACPCALVLSAPATAIAGIA
VAARHGILIRSSAFLEELADLTSLVVDKTGTLTYGVLRLQSIEHAQDAPSSLLLPLAASL
GSASSHPVSRALAGLAEQEDSLALTDIHERQGLGVVAMTAQGEAALGRPELFAQLGIQTS
AVPEHDGPIAGLALDGQFLAWLLLADSVKPEARVAMAELRELGLGRQLLLTGDRQSVATT
LARDVGIGDLQAQALPEDKLKRVMAEIDHGFRPMVVGDGINDSLALKAGVVGVAMGAGGA
DIALASADIVLIGSDLRRLGTCVRLSRQCRRTLQVNVIIGLGWTLAIVAFAAFGWLGAAG
AMIAALLHNLSTLLVLGNAGRLLRFQEPLLKIQAKGAVG