Protein Info for Pf6N2E2_4241 in Pseudomonas fluorescens FW300-N2E2

Annotation: Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase" amino acids 2 to 478 (477 residues), 568.1 bits, see alignment E=7.6e-175 PF00483: NTP_transferase" amino acids 4 to 287 (284 residues), 157.8 bits, see alignment E=7.8e-50 PF12804: NTP_transf_3" amino acids 5 to 135 (131 residues), 30.3 bits, see alignment E=8.8e-11 PF01050: MannoseP_isomer" amino acids 323 to 474 (152 residues), 216.6 bits, see alignment E=3.2e-68 PF07883: Cupin_2" amino acids 390 to 457 (68 residues), 34.7 bits, see alignment E=2.4e-12

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a5630)

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.22

Use Curated BLAST to search for 2.7.7.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A175 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Pf6N2E2_4241 Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) (Pseudomonas fluorescens FW300-N2E2)
VLIPVILSGGAGTRLWPVSREGHPKPFMLLPDGQSLLGKTYRRAAGLFDGRGDIVTVTNR
EYYFQSRDHYQSAHLERHRGHFVLEPTGRNTAPAIATAALAVQALHGDDPILVVMPADHL
IQDEAAFQTAVAHAVELAKNGHLVTFGVLPSTPETGFGYIERGKPLDTKGAAKVVRFVEK
PDLQTATQYLESGRFLWNSGMFCFSVKTLLAELQAHAPELLEQARTCVTASTAVETSGCV
QQELSAAHFAEMPDISIDYALMERSANVVVIPAAFDWSDIGSWSAVAGLVPADAQNNRAT
GEALFIDSQNNFVQSDGRLVAALGVEHLIIIDTADAVLVAHADRAQDVRRVARQLKDKDH
EAYRLHRTVSRPWGSYTVLEEGPRFKIKRIVVKPGASLSLQMHHHRNEHWVVVEGMAKVT
NNGSGSHLVAKNESTFIPAGHKHRLENPGVIDLVIIEVQSGEYLGEDDIVRFEDHYGRAD
SCC