Protein Info for Pf6N2E2_424 in Pseudomonas fluorescens FW300-N2E2

Annotation: Decarboxylase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 PF14793: DUF4478" amino acids 10 to 116 (107 residues), 146.8 bits, see alignment E=4.3e-47 PF03641: Lysine_decarbox" amino acids 196 to 331 (136 residues), 96.6 bits, see alignment E=2.9e-31 PF11892: PpnN_C" amino acids 336 to 456 (121 residues), 185.5 bits, see alignment E=6.9e-59

Best Hits

Swiss-Prot: 57% identical to PPNN_ECO57: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli O157:H7

KEGG orthology group: K06966, (no description) (inferred from 100% identity to pba:PSEBR_a3435)

MetaCyc: 57% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]

Predicted SEED Role

"Decarboxylase family protein"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.4

Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZR71 at UniProt or InterPro

Protein Sequence (457 amino acids)

>Pf6N2E2_424 Decarboxylase family protein (Pseudomonas fluorescens FW300-N2E2)
MNQRHVINASVSPKGSLETLSQREVQQLSEAGSGSIYTLFRQCALAILNTGAHVDNAKTI
LEAYKDFEIRIHQQDRGVRLELLNAPADAFVDGEMIASTREMLFSALRDIVYTENELDSQ
RIDLSSSQGISDYVFHLLRNARTLRPGVEPKIVVCWGGHSINTEEYKYTKKVGHELGLRS
LDICTGCGPGVMKGPMKGATIAHAKQRIHGGRYLGLTEPGIIAAEAPNPIVNELVILPDI
EKRLEAFVRVGHGIIIFPGGAGTAEEFLYLLGILMHPANQDLPFPVVLTGPKSAAPYLDQ
LHAFVGATLGEAAQKHYQIIIDDPAEVARQMTQGLKEVKQFRRERNDAFHFNWLLKIDEG
FQRPFDPTHANMASLGLSRDLPAHELAANLRRAFSGIVAGNVKDKGIRLIEEHGPYEIHG
DAAIMEPLDRLLQAFVAQHRMKLPGGAAYVPCYRVVT