Protein Info for Pf6N2E2_4227 in Pseudomonas fluorescens FW300-N2E2

Annotation: NAD synthetase (EC 6.3.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 278 PF02540: NAD_synthase" amino acids 28 to 271 (244 residues), 205.1 bits, see alignment E=4.8e-65 TIGR00552: NAD+ synthetase" amino acids 30 to 272 (243 residues), 224.2 bits, see alignment E=7.9e-71

Best Hits

Swiss-Prot: 61% identical to NADE_PSEA7: NH(3)-dependent NAD(+) synthetase (nadE) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K01916, NAD+ synthase [EC: 6.3.1.5] (inferred from 96% identity to pba:PSEBR_a5642)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.5

Use Curated BLAST to search for 6.3.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A0Z1 at UniProt or InterPro

Protein Sequence (278 amino acids)

>Pf6N2E2_4227 NAD synthetase (EC 6.3.1.5) (Pseudomonas fluorescens FW300-N2E2)
MTTLVQERIARELGIDRQLTRGGEATEIARRIEFIKQILRESGCKSLVLGISGGVDSLTA
GRLCQLAVEQLREEDYEARFIAVRLPYKAQADEQDAQACLDFIRPDVITTSNIAACVDGL
MGSIAIDGLQPSAELTDFAKGNAKARARMLAQYAIANLSNGLVVGTDHGAEAVMGFFTKF
GDGACDLAPLSGLTKTQVRLLADTLGAPANLVRKAPTADLEDLAPGKLDEVAYGCSYEEI
DGYLMGEVVSPQARQIIERAYLKTAHKRALPRVPPTRL