Protein Info for Pf6N2E2_4217 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 610 (609 residues), 859.1 bits, E=9.2e-263
• PF13522: GATase_6
  • amino acids 67 to 182 (116 residues), 68.5 bits, E=8.8e-23
• PF13537: GATase_7
  • amino acids 83 to 192 (110 residues), 54.5 bits, E=1.8e-18
• PF01380: SIS
  • amino acids 290 to 417 (128 residues), 109.7 bits, E=1.4e-35
  • amino acids 463 to 592 (130 residues), 93 bits, E=2.1e-30

Best Hits

Swiss-Prot: 90% identical to GLMS_PSESM: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 98% identity to pba:PSEBR_a5648)

MetaCyc: 63% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (10/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.16

Sequence Analysis Tools

See A0A160A0Y1 at UniProt or InterPro

Protein Sequence (610 amino acids)

>Pf6N2E2_4217 Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) (Pseudomonas fluorescens FW300-N2E2)
MCGIVGAVAERNITAILLEGLKRLEYRGYDSAGVAVFTNDEKLERMRRPGKVSELEQALE
AEPLLGRLGIAHTRWATHGAPCERNAHPHFSGELAVVHNGIIENHEALREQLKALGYVFT
SDTDTEVIAHLLNHKLKDLVDLTVALKATVKELHGAYGLAVISTKQPDRLVAARSGSPLV
IGLGLGENFLASDQLALRQVTDRFMYLEEGDIAEIRRDSVQIWDLDGNDVVRETVQYRDG
ADVADKGEFRHFMLKEIHEQPAVVQRTLEGRMSQNQVLVQAFGPQAAELFAKVRNVQIVA
CGTSYHAGMVARYWLEELAGIPCQVEVASEFRYRKVVVQPDSLFVTISQSGETADTLAAL
RNAKELGFLASLAICNVGISSLVRESDLTLLTQAGREIGVASTKAFTTQLVGLLLLTLSL
GQVRGTLGKGVEATLVEELRRLPARLGEALAMDSTVEKIAELFAEKNHTLFLGRGAQFPV
AMEGALKLKEISYIHAEAYPAGELKHGPLALVDNDMPVVTVAPNNELLEKLKSNLQEVRA
RGGQLIVFADEKAGMTNGEGTHVVHMPHIHDILSPILYTIPLQLLSYYVAVLKGTDVDQP
RNLAKSVTVE