Protein Info for Pf6N2E2_4190 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 683 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 682 (682 residues), 810 bits, see alignment E=1.3e-247 PF02092: tRNA_synt_2f" amino acids 6 to 541 (536 residues), 687.2 bits, see alignment E=2e-210 PF05746: DALR_1" amino acids 579 to 675 (97 residues), 30.8 bits, see alignment E=2.8e-11

Best Hits

Swiss-Prot: 91% identical to SYGB_PSEF5: Glycine--tRNA ligase beta subunit (glyS) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 100% identity to pba:PSEBR_a11)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H3J2 at UniProt or InterPro

Protein Sequence (683 amino acids)

>Pf6N2E2_4190 Glycyl-tRNA synthetase beta chain (EC 6.1.1.14) (Pseudomonas fluorescens FW300-N2E2)
MSAQDFLVELGTEELPPKALNTLADAFLAGIDKGLQAAGLSYEAKQVYAAPRRLAVLITA
LATQQPDRSINLDGPPRQAAFDADGNPTQAALGFAKKCGVDLSEIDQSGPKLRYSQSIKG
KPTASLLPTIVEDSLNDLPIPKRMRWAARKEEFVRPTQWLVMLLGDQVIDCTILAQKAGR
DSRGHRFHHPQSVRITSPANYLADLRAAYVLADANERRALISKRTEELATLQEGTAIVPA
NLLDEVTALVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDAEGKLLPRFITVA
NIESKDPQQIIAGNEKVVRPRLTDAEFFFKQDKKQPLESFNERLRNVVFQEKLGSVYDKA
ERVSKLAAFIAPRIGGDAQRAARAGILSKCDLSTEMVGEFPEMQGVAGYYYALNDGEPQD
VALALNEQYMPRGAGAELPTTLTGAAVAIADKLDTLVGIFGIGMLPTGSKDPYALRRAAL
GVLRILIDKQLDLDLNDAVAFAVNAFGSKVKSAGLADAVLEFIFDRLRARYEDEGVDVAT
YLSVRALKPGSALDFDQRVQAVQAFRKLPEAAALASVNKRVSNLLSKAEPSVKAVEARYF
DNAAEFSLNSAIQQAYASVQPLMEKRDYAEALARLAALREPVDAFFEAVMVNADNEDVRK
NRYALLARLRSLFLNIADISVLG