Protein Info for Pf6N2E2_418 in Pseudomonas fluorescens FW300-N2E2

Annotation: Signal transduction histidine kinase CheA (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 transmembrane" amino acids 664 to 672 (9 residues), see Phobius details PF01627: Hpt" amino acids 10 to 92 (83 residues), 61.2 bits, see alignment E=1.8e-20 PF02895: H-kinase_dim" amino acids 307 to 349 (43 residues), 35.3 bits, see alignment 2.6e-12 PF02518: HATPase_c" amino acids 412 to 549 (138 residues), 61.9 bits, see alignment E=1.6e-20 PF01584: CheW" amino acids 555 to 681 (127 residues), 99.8 bits, see alignment E=2.2e-32

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 96% identity to pba:PSEBR_a3441)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H9X0 at UniProt or InterPro

Protein Sequence (700 amino acids)

>Pf6N2E2_418 Signal transduction histidine kinase CheA (EC 2.7.3.-) (Pseudomonas fluorescens FW300-N2E2)
VSINLDQAQQTFIVEAREQLQAMEESLLQLETDPGDDDAIGAIFRAAHTIKGSAGLFGLE
PIVSFTHIVEDVLDRLRNGSVEVDAGLIAVLLKSSDHMLELINVAANQGGALPPPALQRE
VELCQILQEYQAPSPATAPTPAQNTETREPGETRLWHISLRFGQDVFRNGMDPLSFLRYL
QTLGEIVDITTLTDALPAADAWDAESCYLGFDIEFRSAAGHGAINEVFDFVREDCEIEIV
AQDDDIAPQANTGLVTTSPEPAEETTALVATGELLSDQRKTPRMPAQIATDKQAVSSDSK
AKDGTYVRVNADKLDELINLVGELVIASAGASLLARTCHNDPLQESTSTVSSLVEEILDG
ALRLRMIPIGETFNRFRRVVRDVSQELGKDIDLIISGAETELDKTVVEKIGDPLMHLLRN
AMDHGIETAEARLAAGKPAKGHLHLNAYHDSGSIVLEIADDGAGLNRDRILEKAQERGLV
SPGASPTDQEIYNLIFEPGFSTAQAVTNLSGRGVGMDVVKRNITLLRGTVDLDSQPGKGT
VVRIRLPLTLAIINGFLVGIGQSTYVIPLDMVQECIELSENDGVASREQGYLDLRGEVLP
LVYLRDHFSHEGPPARRQNVVVVRYAELKAGLVVDDLLGEFQTVIKPLGKLFGALRGISG
STILGSGAVALILDVPALLTQIAQVENRFTSTQLQQANAR