Protein Info for Pf6N2E2_4154 in Pseudomonas fluorescens FW300-N2E2

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 transmembrane" amino acids 213 to 235 (23 residues), see Phobius details PF12792: CSS-motif" amino acids 11 to 205 (195 residues), 92.6 bits, see alignment E=2.5e-30 PF00563: EAL" amino acids 244 to 475 (232 residues), 213.8 bits, see alignment E=2.4e-67

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a47)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZX33 at UniProt or InterPro

Protein Sequence (501 amino acids)

>Pf6N2E2_4154 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens FW300-N2E2)
VVVQQIQQRESDQMNAQGERFLQRLEQLFGQLRESLDDLQNQPLRGCNDDMIATLRQVSF
NYRFVYEAAYIDGSGVCSNRPRQGGLSVTRPPDIRGPTYNYWLNTTTEPDEDRAALMLGR
GNFRVATSRGHLTDVVDLPPGSSLLVVLDNGKRAIPVLGMDQHWPPVEPWPPKSHNALQV
TQNRLIYRMPTESPVYQLVLIAQRNGFKVPANAWWMLPISLMLGLGIGFQVFLLARQRQS
MDAELHGAIRRGELQVLYQPIFDLSSRNCVGAEALLRWRRPDGTLTSPDLFIPMAENTGQ
IRQITDFVLQRLFDQLGQLLRANPQLYISVNLAACDVMVPRIGEVIARLLALHRVSAHQI
AFEVTERGLVDVLVARDNLQSLRDVGHQVLIDDFGTGYCSLAYLQTLPVDCLKIDKAFID
ALGHDAASSGVAPHIIRMAHALQLKVIAEGIEHEAQASYLSSEGVRFGQGWLFAHALSAV
QLIELITRGRRLRGRRLDDEA