Protein Info for Pf6N2E2_4125 in Pseudomonas fluorescens FW300-N2E2

Annotation: Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 transmembrane" amino acids 230 to 250 (21 residues), see Phobius details PF21102: DprA_N" amino acids 15 to 47 (33 residues), 26.1 bits, see alignment 8.1e-10 TIGR00732: DNA protecting protein DprA" amino acids 78 to 297 (220 residues), 261.6 bits, see alignment E=2.1e-82 PF02481: DNA_processg_A" amino acids 88 to 297 (210 residues), 256.4 bits, see alignment E=2.3e-80 PF17782: WHD_DprA" amino acids 304 to 354 (51 residues), 38.5 bits, see alignment 1.5e-13

Best Hits

KEGG orthology group: K04096, DNA processing protein (inferred from 98% identity to pba:PSEBR_a72)

Predicted SEED Role

"Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A0Q7 at UniProt or InterPro

Protein Sequence (364 amino acids)

>Pf6N2E2_4125 Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake (Pseudomonas fluorescens FW300-N2E2)
MSLPESTPVSPAELEARLRLHRLPELGPKRFMTLMEAFGSASKAVSAPASAWRALGLPLA
CVEARRNPDVRDGAAHALAWLERAGQHLLMWDQPDYPALLAQISDAPPLLFVAGDASILE
KPQLAMVGSRRASRPGMDTAAAFSRSLAGAGFVITSGLALGIDAAAHQAALDVGGRTVGV
LGTGLENFYPQRNRRLADAMIAQGSAVLSEFPLDAPPHASNFPRRNRIISGLSLGVLVVE
ASVASGSLITARLAAEQGREVYAIPGSIHHPGARGCHQLIRDGAALVETVEHILEALRGW
QRLPPASEPVPVTHPLLRLLHAAPLSSEALAEASGWGLPKVLAALTELEMDGRAICDNGR
WFAR