Protein Info for Pf6N2E2_4087 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): L-asparaginase (EC 3.5.1.1)
Rationale: Specifically important for utilizing L-Asparagine. Automated validation from mutant phenotype: the predicted function (ASPARAGHYD-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: L-asparaginase (EC 3.5.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00710: Asparaginase
  • amino acids 11 to 183 (173 residues), 140.2 bits, E=5.9e-45
• PF17763: Asparaginase_C
  • amino acids 209 to 324 (116 residues), 94.5 bits, E=4.4e-31

Best Hits

KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 97% identity to pba:PSEBR_a107)

Predicted SEED Role

"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)

MetaCyc Pathways

• L-asparagine degradation I (1/1 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
• L-asparagine degradation III (mammalian) (2/3 steps found)
• N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Cyanoamino acid metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.1

Sequence Analysis Tools

See A0A161H3I1 at UniProt or InterPro

Protein Sequence (334 amino acids)

>Pf6N2E2_4087 L-asparaginase (EC 3.5.1.1) (Pseudomonas fluorescens FW300-N2E2)
MNGSTYPAAQHVMVLYTGGTIGMQASAHGLAPASGFEARMRDYLHSQPELVVPQWRFREM
SPLIDSANMTPAYWQQLREAVVDAVDVQGCDSVLILHGTDTLAYSAAAMSFQLLGLHARV
CFTGSMLPAGVIDSDAWENLSGALVALGQGLAPGVHLYFHGELLAPTRCAKVRSFGRHPF
KRLERQGGGLKASSLPAQLNYNQPKQLANIAALPLFPGISAEILDGLLGSGIQGLVLECY
GSGTGPSDNPAFLASLERARDSGVVIVAVTQCHEGGVELDVYEAGSRLRGVGVLSGGGMT
REAAFGKLQALIGAGLPVEEVRRLVELDLCGELA