Protein Info for Pf6N2E2_4038 in Pseudomonas fluorescens FW300-N2E2

Annotation: NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF05222: AlaDh_PNT_N" amino acids 39 to 171 (133 residues), 140.2 bits, see alignment E=5.4e-45 PF01262: AlaDh_PNT_C" amino acids 175 to 405 (231 residues), 230.4 bits, see alignment E=1.7e-72

Best Hits

Swiss-Prot: 53% identical to PNTAA_RHORU: NAD(P) transhydrogenase subunit alpha part 1 (pntAA) from Rhodospirillum rubrum

KEGG orthology group: K00324, NAD(P) transhydrogenase subunit alpha [EC: 1.6.1.2] (inferred from 99% identity to pba:PSEBR_a154)

Predicted SEED Role

"NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A0W9 at UniProt or InterPro

Protein Sequence (408 amino acids)

>Pf6N2E2_4038 NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2) (Pseudomonas fluorescens FW300-N2E2)
MPRPAWFGGIVLIGRPSRTIRSAWVYSIRSRRRFDVHIGVPLETQTGETRVAATPETIKK
LIGQGHKVTVQSGAGIKASVIDSAYEAAGATIGSASDAFGAELILKVVAPSDSELTLIKS
GTVLVGMLNPFNNETIAKLAERGITAFALEAAPRTSRAQSLDVLSSQANIAGYKAVLLAA
HHYPRFMPMLMTAAGTVKAARVLILGAGVAGLQAIATAKRLGAVIEASDVRPAVKEQIES
LGAKFVDVPYETDEERECAVGVGGYARPMPASWMQRQAQAVHERAKQADIVITTALIPGR
KAPTLLSAETVAQMKPGSVVIDLAAAQGGNCPLTVADQVVIENGVTICGPTNLAGEVAAD
ASALYARNLLDFLKLVFTKEGQFDVNLEDDIVAACLMCRDGQVIRKNA